CoraLite® Plus 488-conjugated BCL6 Recombinant antibody

BCL6 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3K6

Cat no : CL488-81754

Print datasheet

Synonyms

B cell CLL/lymphoma 6, B cell lymphoma 5 protein, B cell lymphoma 6 protein, BCL6, LAZ3, Protein LAZ 3, ZBTB27, Zinc finger protein 51, ZNF51



经过测试的应用

Positive FC (Intra) detected inRamos cells
Positive FC detected inRamos cells
For other applications, we recommend the unconjugated version of this antibody, 81754-1-PBS

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-81754 targets BCL6 in FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human
Immunogen BCL6 fusion protein Ag15519 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name B-cell CLL/lymphoma 6
Synonyms B cell CLL/lymphoma 6, B cell lymphoma 5 protein, B cell lymphoma 6 protein, BCL6, LAZ3, Protein LAZ 3, ZBTB27, Zinc finger protein 51, ZNF51
Calculated Molecular Weight 706 aa, 79 kDa
Observed Molecular Weight 79-95 kDa
GenBank Accession NumberBC150184
Gene Symbol BCL6
Gene ID (NCBI) 604
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP41182
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 BCL6 antibody CL488-81754Download protocol
Standard Protocols
Click here to view our Standard Protocols