验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | Ramos cells |
| Positive FC detected in | Ramos cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-81754 targets BCL6 in FC (Intra) applications and shows reactivity with Human samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | Human |
| Immunogen | BCL6 fusion protein Ag15519 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | B-cell CLL/lymphoma 6 |
| Synonyms | B cell CLL/lymphoma 6, B cell lymphoma 5 protein, B cell lymphoma 6 protein, BCL6, LAZ3, Protein LAZ 3, ZBTB27, Zinc finger protein 51, ZNF51 |
| Calculated Molecular Weight | 706 aa, 79 kDa |
| Observed Molecular Weight | 79-95 kDa |
| GenBank Accession Number | BC150184 |
| Gene Symbol | BCL6 |
| Gene ID (NCBI) | 604 |
| RRID | AB_3084506 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P41182 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCL6, a zinc finger transcription factor, contains an N-terminal BTB/POZ domain and C-terminal zinc finger DNA-binding motifs and represses transcription of a wide range of target proteins and microRNAs. BCL6 protein has been reported as a master regulator of B lymphocyte development and growth, and altered BCL6 protein expression was implicated in pathogenesis of diverse human hematologic malignancies, especially in the diffuse large B cell lymphoma (DLBCL). BCL6 is required for the development of T follicular helper T cells (TFH), a helper T cell subset required for the formation of mature and productive GCs. BCL6 has also been shown to play important regulatory roles in macrophages.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 BCL6 antibody CL488-81754 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |