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验证数据展示

  • FC experiment of HepG2 using CL488-67860

    FC experiment of HepG2 using CL488-67860

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human BCLAF1 (CL488-67860, Clone:1D6G7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated BCLAF1 Monoclonal antibody

BCLAF1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67860

产品说明书

CloneNo. 1D6G7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

FC (Intra)

别名

KIAA0164, Btf, BCLAF1 and THRAP3 family member 1, Bcl-2-associated transcription factor 1, 1D6G7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy BCLAF1 Ready-To-Use IHC Kit. BCLAF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67860-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67860 targets BCLAF1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 BCLAF1 fusion protein Ag25210 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 BCL2-associated transcription factor 1
别名 KIAA0164, Btf, BCLAF1 and THRAP3 family member 1, Bcl-2-associated transcription factor 1, 1D6G7
计算分子量 920 aa, 106 kDa
观测分子量145 kDa
GenBank蛋白编号BC132780
基因名称 BCLAF1
Gene ID (NCBI) 9774
RRIDAB_2923808
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NYF8
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCLF1, also named as Bcl-2-associated transcription factor 1, is a 920 amino acid protein, which Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA. BCLF1 as a death-promoting transcriptional repressor may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. The calculated molecular weight of BCLF1 is a 110 kDa, but the modified BCLF1 protein is about 140-150 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 BCLAF1 antibody CL488-67860Download protocol
Standard Protocols
Click here to view our Standard Protocols