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BDH1 Monoclonal antibody
BDH1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig and More (2)
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
2C3D4
验证数据展示
经过测试的应用
| Positive WB detected in | pig liver tissue, HSC-T6 cells, HepG2 cells, HT-29 cells, COLO 320 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
Planning an IHC experiment? We recommend our IHCeasy BDH1 Ready-To-Use IHC Kit. BDH1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
67448-1-Ig targets BDH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Applications | WB, IHC, IF-P, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Cited Reactivity | human, mouse |
| Immunogen | BDH1 fusion protein Ag7864 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | 3-hydroxybutyrate dehydrogenase, type 1 |
| Synonyms | BDH, BDH1, SDR9C1 |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC005844 |
| Gene Symbol | BDH1 |
| Gene ID (NCBI) | 622 |
| RRID | AB_2882682 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q02338 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BDH1 antibody 67448-1-Ig | Download protocol |
| IHC protocol for BDH1 antibody 67448-1-Ig | Download protocol |
| IF protocol for BDH1 antibody 67448-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
MedComm (2020) BDH1-mediated LRRC31 regulation dependent on histone lysine β-hydroxybutyrylation to promote lung adenocarcinoma progression
| ||
