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  • Featured Product
  • KD/KO Validated

BDH1 Monoclonal antibody, PBS Only

BDH1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2C3D4

Cat no : 67448-1-PBS

Print datasheet

Synonyms

SDR9C1, EC:1.1.1.30, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, BDH, 3-hydroxybutyrate dehydrogenase

验证数据展示

  • WB analysis of pig liver using 67448-1-Ig (same clone as 67448-1-PBS)

    WB analysis of pig liver using 67448-1-Ig (same clone as 67448-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 67448-1-Ig (same clone as 67448-1-PBS)

    WB analysis of HSC-T6 using 67448-1-Ig (same clone as 67448-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67448-1-Ig (BDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67448-1-Ig (same clone as 67448-1-PBS)

    IHC staining of human liver cancer using 67448-1-Ig (same clone as 67448-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67448-1-Ig (BDH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BDH1 antibody included
  • IHC staining of human liver cancer using 67448-1-Ig (same clone as 67448-1-PBS)

    IHC staining of human liver cancer using 67448-1-Ig (same clone as 67448-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67448-1-Ig (BDH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BDH1 antibody included
  • IF Staining of mouse liver using 67448-1-Ig (same clone as 67448-1-PBS)

    IF Staining of mouse liver using 67448-1-Ig (same clone as 67448-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BDH1 antibody (67448-1-Ig, Clone: 2C3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 67448-1-Ig (same clone as 67448-1-PBS)

    IF Staining of mouse liver using 67448-1-Ig (same clone as 67448-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BDH1 antibody (67448-1-Ig, Clone: 2C3D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67448-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy BDH1 Ready-To-Use IHC Kit. BDH1 primary antibody included.

产品信息

67448-1-PBS targets BDH1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen BDH1 fusion protein Ag7864 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name 3-hydroxybutyrate dehydrogenase, type 1
Synonyms SDR9C1, EC:1.1.1.30, D-beta-hydroxybutyrate dehydrogenase, mitochondrial, BDH, 3-hydroxybutyrate dehydrogenase
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC005844
Gene Symbol BDH1
Gene ID (NCBI) 622
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02338
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).