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BDNF Monoclonal antibody, PBS Only

BDNF Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E5C5

Cat no : 66292-1-PBS

Print datasheet

Synonyms

Abrineurin, BDNF

验证数据展示

  • WB analysis of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    WB analysis of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 66292-1-Ig (BDNF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    IF Staining of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

产品信息

66292-1-PBS targets BDNF in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen BDNF fusion protein Ag11329 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name brain-derived neurotrophic factor
Synonyms Abrineurin, BDNF
Calculated Molecular Weight 247 aa, 28 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC029795
Gene Symbol BDNF
Gene ID (NCBI) 627
RRIDAB_2881675
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23560
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).