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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    WB analysis of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    U-251 cells were subjected to SDS PAGE followed by western blot with 66292-1-Ig (BDNF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    IF Staining of U-251 using 66292-1-Ig (same clone as 66292-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using BDNF antibody (66292-1-Ig, Clone: 1E5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66292-1-PBS in a different storage buffer formulation.

BDNF Monoclonal antibody, PBS Only

BDNF Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66292-1-PBS

Print datasheet

CloneNo. 1E5C5

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF precursor form, 1E5C5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

66292-1-PBS targets BDNF in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen BDNF fusion protein Ag11329 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name brain-derived neurotrophic factor
Synonyms Brain-derived neurotrophic factor, brain derived neurotrophic factor, BDNF precursor form, 1E5C5
Calculated Molecular Weight 247 aa, 28 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC029795
Gene Symbol BDNF
Gene ID (NCBI) 627
RRIDAB_2881675
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23560
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

1) What is BDNF?

BDNF (brain-derived neurotrophic factor) is a small secreted growth factor that is important for the development and plasticity of the central nervous system and vital for long-term memory. Selected polymorphisms of BDNF have been shown to increase susceptibility to memory impairment and selected eating and mental disorders.

2) FAQs for BDNF

A) I can detect more than one band in my samples
BDNF is a neurotrophin that is a subject of maturation by proteolytic cleavage. The precursor protein (pre-proBDNF) is first cleaved to proBDNF (~34 kDa) by removing a signal peptide, and then to a mature form of BDNF (14 kDa). Additionally, (pre-)proBDNF can form dimers that run between 50 and 60 kDa, and a minor truncated form of proBDNF running at 28 kDa has also been reported (PMID: 11152678).