• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

BID Recombinant antibody, PBS Only

BID Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242460F1

Cat no : 84944-4-PBS

Print datasheet

Synonyms

p22 BID, BH3-interacting domain death agonist p15, BH3-interacting domain death agonist p13, BH3-interacting domain death agonist p11, BH3-interacting domain death agonist

验证数据展示

  • WB analysis using 84944-4-RR (same clone as 84944-4-PBS)

    WB analysis using 84944-4-RR (same clone as 84944-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84944-4-RR (BID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84944-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84944-4-RR (same clone as 84944-4-PBS)

    FC experiment of HeLa using 84944-4-RR (same clone as 84944-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BID Recombinant antibody (84944-4-RR, Clone:242460F1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84944-4-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 84944-4-RR (same clone as 84944-4-PBS)

    FC experiment of MCF-7 using 84944-4-RR (same clone as 84944-4-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug BID Recombinant antibody (84944-4-RR, Clone:242460F1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84944-4-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy BID Ready-To-Use IHC Kit. BID primary antibody included.

产品信息

84944-4-PBS targets BID in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name BH3 interacting domain death agonist
Synonyms p22 BID, BH3-interacting domain death agonist p15, BH3-interacting domain death agonist p13, BH3-interacting domain death agonist p11, BH3-interacting domain death agonist
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC009197
Gene Symbol BID
Gene ID (NCBI) 637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP55957
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606)