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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 84944-4-RR (same clone as 84944-4-PBS)

    WB analysis using 84944-4-RR (same clone as 84944-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84944-4-RR (BID antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84944-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84944-4-RR (same clone as 84944-4-PBS)

    FC experiment of HeLa using 84944-4-RR (same clone as 84944-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BID Recombinant antibody (84944-4-RR, Clone:242460F1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84944-4-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 84944-4-RR (same clone as 84944-4-PBS)

    FC experiment of MCF-7 using 84944-4-RR (same clone as 84944-4-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug BID Recombinant antibody (84944-4-RR, Clone:242460F1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84944-4-PBS in a different storage buffer formulation.

BID Recombinant antibody, PBS Only

BID Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84944-4-PBS

产品说明书

CloneNo. 242460F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

p22 BID, BH3-interacting domain death agonist p15, BH3-interacting domain death agonist p13, BH3-interacting domain death agonist p11, BH3-interacting domain death agonist

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy BID Ready-To-Use IHC Kit. BID primary antibody included.

产品信息

84944-4-PBS targets BID in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BH3 interacting domain death agonist
别名 p22 BID, BH3-interacting domain death agonist p15, BH3-interacting domain death agonist p13, BH3-interacting domain death agonist p11, BH3-interacting domain death agonist
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC009197
基因名称 BID
Gene ID (NCBI) 637
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP55957
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606)