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验证数据展示

  • IF Staining of mouse brain using CL488-14647

    IF Staining of mouse brain using CL488-14647

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 BIN1 antibody (CL488-14647) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated BIN1 Polyclonal antibody

BIN1 Polyclonal Antibody for IF-P
Cat No. CL488-14647

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

AMPH2, Amphiphysin II, Amphiphysin like protein, AMPHL, BIN1, bridging integrator 1, DKFZp547F068, SH3P9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy BIN1 Ready-To-Use IHC Kit. BIN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14647-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14647 targets BIN1 in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 BIN1 fusion protein Ag6240 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bridging integrator 1
别名 AMPH2, Amphiphysin II, Amphiphysin like protein, AMPHL, BIN1, bridging integrator 1, DKFZp547F068, SH3P9
计算分子量 65 kDa
观测分子量 50-65 kDa
GenBank蛋白编号BC004101
基因名称 BIN1
Gene ID (NCBI) 274
RRIDAB_3672597
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00499
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BIN1 (Bridging integrator 1), also known as amphiphysin II or Myc box-dependent-interacting protein 1, is a ubiquitous nucleocytoplasmic adaptor protein that was identified initially as an MYC-interacting proapoptotic tumor suppressor. Alternative splicing of the gene results in multiple transcript variants encoding different isoforms. BIN1 is a key regulator of different cellular functions, including endocytosis and membrane recycling, cytoskeleton regulation, DNA repair, cell cycle progression, and apoptosis (PMID: 24590001).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BIN1 antibody CL488-14647Download protocol
Standard Protocols
Click here to view our Standard Protocols