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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated BIN1 Polyclonal antibody

BIN1 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14647

Print datasheet

Synonyms

AMPH2, Amphiphysin II, Amphiphysin like protein, AMPHL, BIN1, bridging integrator 1, DKFZp547F068, SH3P9

验证数据展示

  • IF Staining of mouse brain using CL488-14647

    IF Staining of mouse brain using CL488-14647

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CoraLite® Plus 488 BIN1 antibody (CL488-14647) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy BIN1 Ready-To-Use IHC Kit. BIN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14647-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14647 targets BIN1 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen BIN1 fusion protein Ag6240 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bridging integrator 1
Synonyms AMPH2, Amphiphysin II, Amphiphysin like protein, AMPHL, BIN1, bridging integrator 1, DKFZp547F068, SH3P9
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 50-65 kDa
GenBank Accession NumberBC004101
Gene Symbol BIN1
Gene ID (NCBI) 274
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00499
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BIN1 (Bridging integrator 1), also known as amphiphysin II or Myc box-dependent-interacting protein 1, is a ubiquitous nucleocytoplasmic adaptor protein that was identified initially as an MYC-interacting proapoptotic tumor suppressor. Alternative splicing of the gene results in multiple transcript variants encoding different isoforms. BIN1 is a key regulator of different cellular functions, including endocytosis and membrane recycling, cytoskeleton regulation, DNA repair, cell cycle progression, and apoptosis (PMID: 24590001).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BIN1 antibody CL488-14647Download protocol
Standard Protocols
Click here to view our Standard Protocols