• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

BIN3 Polyclonal antibody

BIN3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11215-1-AP

Print datasheet

Synonyms

BIN3, bridging integrator 3

验证数据展示

  • WB analysis of K-562 using 11215-1-AP

    WB analysis of K-562 using 11215-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11215-1-AP (BIN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11215-1-AP

    IHC staining of human prostate cancer using 11215-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11215-1-AP (BIN3 antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of K-562 using 11215-1-AP

    IP experiment of K-562 using 11215-1-AP

    IP result of anti-BIN3 (IP:11215-1-AP, 4ug; Detection:11215-1-AP 1:300) with K-562 cells lysate 3200ug.

经过测试的应用

Positive WB detected inK-562 cells, HeLa cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11215-1-AP targets BIN3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen BIN3 fusion protein Ag1713 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bridging integrator 3
Synonyms BIN3, bridging integrator 3
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC009824
Gene Symbol BIN3
Gene ID (NCBI) 55909
RRIDAB_2063649
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQY0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Bridging integrator 3 (BIN3) is a conserved BAR (Bin/amphiphysin/ Rvs) domain adaptor protein (PMID: 11274158). BAR domain proteins, sharing an N-terminal BAR domain, have been implicated in diverse cellular processes, including endocytosis, vesicle fusion, cell polarity, actin organization, signal transduction, transcription repression, and tumor suppression (PMID: 16524918). BIN3 is involved in cytokinesis and septation where it has a role in the localization of F-actin (PMID: 11274158).

实验方案

Product Specific Protocols
WB protocol for BIN3 antibody 11215-1-APDownload protocol
IHC protocol for BIN3 antibody 11215-1-APDownload protocol
IP protocol for BIN3 antibody 11215-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Med

Implication of the miR-184 and miR-204 competitive RNA network in control of mouse secondary cataract.

Authors - Hoffmann Andrea A
View Article