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BLM Polyclonal antibody

BLM Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30254-1-AP

Print datasheet

Synonyms

BLM, Bloom syndrome protein, BS, DNA helicase, RecQ like type 2, RecQ protein like 3, RECQ2, RECQL2, RECQL3

验证数据展示

  • WB analysis using 30254-1-AP

    WB analysis using 30254-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30254-1-AP (BLM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30254-1-AP

    WB analysis using 30254-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30254-1-AP (BLM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNIH/3T3 cells, HEK-293T cells, HeLa cells, Jurkat cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

30254-1-AP targets BLM in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen BLM fusion protein Ag32996 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Bloom syndrome, RecQ helicase-like
Synonyms BLM, Bloom syndrome protein, BS, DNA helicase, RecQ like type 2, RecQ protein like 3, RECQ2, RECQL2, RECQL3
Calculated Molecular Weight159 kDa
Observed Molecular Weight150-159 kDa
Gene Symbol BLM
Gene ID (NCBI) 641
RRIDAB_3086282
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP54132
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BLM participates in DNA replication and repair, it is involved in 5'-end resection of DNA during double-strand break (DSB) repair. BLM can also be recruited by the KHDC3L-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks.

实验方案

Product Specific Protocols
WB protocol for BLM antibody 30254-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

YY1 is involved in homologous recombination inhibition at guanine quadruplex sites in human cells

Authors - Xinyu Cui
View Article