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BLNK Monoclonal antibody, PBS Only

BLNK Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1A1

Cat no : 66953-1-PBS

Print datasheet

Synonyms

B cell linker, B cell linker protein, BASH, BLNK, BLNK S, Cytoplasmic adapter protein, LY57, SLP 65, SLP65

验证数据展示

  • WB analysis of human spleen using 66953-1-Ig (same clone as 66953-1-PBS)

    WB analysis of human spleen using 66953-1-Ig (same clone as 66953-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 66953-1-Ig (same clone as 66953-1-PBS)

    IHC staining of human appendicitis using 66953-1-Ig (same clone as 66953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BLNK antibody included
  • IF Staining of Raji using 66953-1-Ig (same clone as 66953-1-PBS)

    IF Staining of Raji using 66953-1-Ig (same clone as 66953-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using BLNK antibody (66953-1-Ig, Clone: 1A1A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 66953-1-Ig (same clone as 66953-1-PBS)

    IHC staining of human appendicitis using 66953-1-Ig (same clone as 66953-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66953-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BLNK antibody included
Planning an IHC experiment? We recommend our IHCeasy BLNK Ready-To-Use IHC Kit. BLNK primary antibody included.

产品信息

66953-1-PBS targets BLNK in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, pig, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, pig, mouse, rat
Immunogen BLNK fusion protein Ag28673 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name B-cell linker
Synonyms B cell linker, B cell linker protein, BASH, BLNK, BLNK S, Cytoplasmic adapter protein, LY57, SLP 65, SLP65
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC018906
Gene Symbol BLNK
Gene ID (NCBI) 29760
RRIDAB_2882276
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8WV28
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BLNK (also known as SLP-65 or BASH) is an important adaptor protein selectively expressed in B-lineage cells. BLNK bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. BLNK has been shown to be necessary for BCR-mediated Ca2+ mobilization, for the activation of mitogen-activated protein kinases such as ERK, JNK, and p38 in a chicken B cell line DT40, and for activation of transcription factors such as NF-AT and NF-κB in human or mouse B cells. BLNK plays a crucial role in pre-BCR-dependent progression of B cell development, BCR-mediated B cell survival, activation, proliferation, and T-independent immune responses.