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验证数据展示

  • WB analysis of human spleen using 66953-1-Ig

    WB analysis of human spleen using 66953-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66953-1-Ig (BLNK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human appendicitis using 66953-1-Ig

    IHC staining of human appendicitis using 66953-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BLNK antibody included
  • IF Staining of Raji using 66953-1-Ig

    IF Staining of Raji using 66953-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using BLNK antibody (66953-1-Ig, Clone: 1A1A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of human appendicitis using 66953-1-Ig

    IHC staining of human appendicitis using 66953-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66953-1-Ig (BLNK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BLNK antibody included

BLNK Monoclonal antibody

BLNK Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66953-1-Ig

产品说明书

CloneNo. 1A1A1

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

B cell linker, B cell linker protein, BASH, BLNK, BLNK S, Cytoplasmic adapter protein, LY57, SLP 65, SLP65

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman spleen tissue, Raji cells, Daudi cells, Ramos cells, pig spleen tissue
Positive IHC detected inhuman appendicitis tissue, mouse thymus tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy BLNK Ready-To-Use IHC Kit. BLNK primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:3000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66953-1-Ig targets BLNK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, pig, mouse, rat
免疫原 BLNK fusion protein Ag28673 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 B-cell linker
别名 B cell linker, B cell linker protein, BASH, BLNK, BLNK S, Cytoplasmic adapter protein, LY57, SLP 65, SLP65
计算分子量 50 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC018906
基因名称 BLNK
Gene ID (NCBI) 29760
RRIDAB_2882276
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ8WV28
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BLNK (also known as SLP-65 or BASH) is an important adaptor protein selectively expressed in B-lineage cells. BLNK bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. BLNK has been shown to be necessary for BCR-mediated Ca2+ mobilization, for the activation of mitogen-activated protein kinases such as ERK, JNK, and p38 in a chicken B cell line DT40, and for activation of transcription factors such as NF-AT and NF-κB in human or mouse B cells. BLNK plays a crucial role in pre-BCR-dependent progression of B cell development, BCR-mediated B cell survival, activation, proliferation, and T-independent immune responses.

实验方案

Product Specific Protocols
WB protocol for BLNK antibody 66953-1-IgDownload protocol
IHC protocol for BLNK antibody 66953-1-IgDownload protocol
IF protocol for BLNK antibody 66953-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols