验证数据展示
经过测试的应用
| Positive IF/ICC detected in | NIH/3T3 cells |
| Positive FC (Intra) detected in | Daudi cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66953 targets BLNK in IF/ICC, FC (Intra) applications and shows reactivity with human, pig samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, pig |
| 免疫原 | BLNK fusion protein Ag28673 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | B-cell linker |
| 别名 | SLP65, Cytoplasmic adapter protein, B-cell linker protein, B-cell adapter containing a Src homology 2 domain protein, B-cell adapter containing a SH2 domain protein |
| 计算分子量 | 50 kDa |
| 观测分子量 | 65-70 kDa |
| GenBank蛋白编号 | BC018906 |
| 基因名称 | BLNK |
| Gene ID (NCBI) | 29760 |
| RRID | AB_3084738 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q8WV28 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BLNK (also known as SLP-65 or BASH) is an important adaptor protein selectively expressed in B-lineage cells. BLNK bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. BLNK has been shown to be necessary for BCR-mediated Ca2+ mobilization, for the activation of mitogen-activated protein kinases such as ERK, JNK, and p38 in a chicken B cell line DT40, and for activation of transcription factors such as NF-AT and NF-κB in human or mouse B cells. BLNK plays a crucial role in pre-BCR-dependent progression of B cell development, BCR-mediated B cell survival, activation, proliferation, and T-independent immune responses.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 BLNK antibody CL594-66953 | Download protocol |
| FC protocol for CL594 BLNK antibody CL594-66953 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |