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BMPR2 Monoclonal antibody

BMPR2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C1D7

Cat no : 68497-1-Ig

Print datasheet

Synonyms

BMP type 2 receptor, BMP type II receptor, BMPR 2, BMPR II, BMPR2, BMPR3, BMR2, BRK 3, FLJ41585, FLJ76945, PPH1, T ALK

验证数据展示

  • WB analysis using 68497-1-Ig

    WB analysis using 68497-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68497-1-Ig (BMPR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 68497-1-Ig

    WB analysis of NIH/3T3 using 68497-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68497-1-Ig (BMPR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, NIH/3T3 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68497-1-Ig targets BMPR2 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen BMPR2 fusion protein Ag5868 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name bone morphogenetic protein receptor, type II (serine/threonine kinase)
Synonyms BMP type 2 receptor, BMP type II receptor, BMPR 2, BMPR II, BMPR2, BMPR3, BMR2, BRK 3, FLJ41585, FLJ76945, PPH1, T ALK
Calculated Molecular Weight 115 kDa
Observed Molecular Weight120-140 kDa
GenBank Accession NumberBC052985
Gene Symbol BMPR2
Gene ID (NCBI) 659
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13873
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMPs (bone morphogenetic protein) are involved in endochondral bone formation and embryogenesis. BMPR2 encodes a member of the (BMP) receptor family of transmembrane serine/threonine kinases. It is a widely expressed receptor, with high mRNA expression during development in many tissues. Dimer of BMPR2 receptors (70-80 kD) forms a complex with a dimer of type I BMPR1(50-55 kD) in signal transduction. This antibody recognizes endogenous levels of total BMPR2 protein, including 115 kD precursor, 75 kD mature form and 150 kD dimer.

实验方案

Product Specific Protocols
WB protocol for BMPR2 antibody 68497-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols