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验证数据展示

  • WB analysis using 68497-1-Ig

    WB analysis using 68497-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68497-1-Ig (BMPR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 68497-1-Ig

    WB analysis of NIH/3T3 using 68497-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68497-1-Ig (BMPR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

BMPR2 Monoclonal antibody

BMPR2 Monoclonal Antibody for WB, ELISA
Cat No. 68497-1-Ig

产品说明书

CloneNo. 2C1D7

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse

应用

WB, ELISA

别名

BMP type 2 receptor, BMP type II receptor, BMPR 2, BMPR II, BMPR2, BMPR3, BMR2, BRK 3, FLJ41585, FLJ76945, PPH1, T ALK

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, NIH/3T3 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68497-1-Ig targets BMPR2 in WB, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse
免疫原 BMPR2 fusion protein Ag5868 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 bone morphogenetic protein receptor, type II (serine/threonine kinase)
别名 BMP type 2 receptor, BMP type II receptor, BMPR 2, BMPR II, BMPR2, BMPR3, BMR2, BRK 3, FLJ41585, FLJ76945, PPH1, T ALK
计算分子量 115 kDa
观测分子量120-140 kDa
GenBank蛋白编号BC052985
基因名称 BMPR2
Gene ID (NCBI) 659
RRIDAB_3085208
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13873
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BMPs (bone morphogenetic protein) are involved in endochondral bone formation and embryogenesis. BMPR2 encodes a member of the (BMP) receptor family of transmembrane serine/threonine kinases. It is a widely expressed receptor, with high mRNA expression during development in many tissues. Dimer of BMPR2 receptors (70-80 kD) forms a complex with a dimer of type I BMPR1(50-55 kD) in signal transduction. This antibody recognizes endogenous levels of total BMPR2 protein, including 115 kD precursor, 75 kD mature form and 150 kD dimer.

实验方案

Product Specific Protocols
WB protocol for BMPR2 antibody 68497-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols