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验证数据展示

  • IF Staining of HepG2 using CL488-68091

    IF Staining of HepG2 using CL488-68091

    Immunofluorescent analysis of (-20°C Methanol) fixed Cobalt Chloride treated HepG2 cells using CoraLite® Plus 488 BNIP3 antibody (CL488-68091, Clone: 2H7D8 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated BNIP3 Monoclonal antibody

BNIP3 Monoclonal Antibody for IF/ICC
Cat No. CL488-68091

产品说明书

CloneNo. 2H7D8

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

IF/ICC

别名

BNIP3, NIP3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inCobalt Chloride treated HepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 68091-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68091 targets BNIP3 in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 BNIP3 fusion protein Ag27935 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 BCL2/adenovirus E1B 19kDa interacting protein 3
别名 BNIP3, NIP3
计算分子量 194 aa, 22 kDa
观测分子量25-30,50-60 kDa
GenBank蛋白编号BC009342
基因名称 BNIP3
Gene ID (NCBI) 664
RRIDAB_3084396
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ12983
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BNIP3 antibody CL488-68091Download protocol
Standard Protocols
Click here to view our Standard Protocols