• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 18017-1-AP

    WB analysis using 18017-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18017-1-AP (BOLA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 18017-1-AP

    IHC staining of human lung using 18017-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung using 18017-1-AP

    IHC staining of human lung using 18017-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HepG2 using 18017-1-AP

    IP experiment of HepG2 using 18017-1-AP

    IP result of anti-BOLA1 (IP:18017-1-AP, 4ug; Detection:18017-1-AP 1:800) with HepG2 cells lysate 3480ug.

  • IF Staining of HepG2 using 18017-1-AP

    IF Staining of HepG2 using 18017-1-AP

    Immunofluorescent analysis of HepG2 cells using 18017-1-AP (BOLA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

BOLA1 Polyclonal antibody

BOLA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 18017-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

bolA homolog 1 (E. coli), BolA like protein 1, BOLA1, CGI 143, hBolA, RP11 196G18.18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung tissue, human heart tissue, human kidney tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

18017-1-AP targets BOLA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 BOLA1 fusion protein Ag12565 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bolA homolog 1 (E. coli)
别名 bolA homolog 1 (E. coli), BolA like protein 1, BOLA1, CGI 143, hBolA, RP11 196G18.18
计算分子量 137 aa, 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC063405
基因名称 BOLA1
Gene ID (NCBI) 51027
RRIDAB_2066933
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y3E2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BOLA1 antibody 18017-1-APDownload protocol
IHC protocol for BOLA1 antibody 18017-1-APDownload protocol
IF protocol for BOLA1 antibody 18017-1-APDownload protocol
IP protocol for BOLA1 antibody 18017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biomed Res Int

Prognostic Values of BolA Family Member Expression in Hepatocellular Carcinoma

Authors - Dong Wang
View Article
mouseWB

bioRxiv

Proteome profiling identifies a link between the mitochondrial pathways and host-microbial sensor ELMO1 following Salmonella infection

Authors - Sajan C Achi
View Article