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BOLA1 Polyclonal antibody

BOLA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18017-1-AP

Print datasheet

Synonyms

bolA homolog 1 (E. coli), BolA like protein 1, BOLA1, CGI 143, hBolA, RP11 196G18.18

验证数据展示

  • WB analysis using 18017-1-AP

    WB analysis using 18017-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18017-1-AP (BOLA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung using 18017-1-AP

    IHC staining of human lung using 18017-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung using 18017-1-AP

    IHC staining of human lung using 18017-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18017-1-AP (BOLA1 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HepG2 using 18017-1-AP

    IP experiment of HepG2 using 18017-1-AP

    IP result of anti-BOLA1 (IP:18017-1-AP, 4ug; Detection:18017-1-AP 1:800) with HepG2 cells lysate 3480ug.

  • IF Staining of HepG2 using 18017-1-AP

    IF Staining of HepG2 using 18017-1-AP

    Immunofluorescent analysis of HepG2 cells using 18017-1-AP (BOLA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung tissue, human heart tissue, human kidney tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

18017-1-AP targets BOLA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen BOLA1 fusion protein Ag12565 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bolA homolog 1 (E. coli)
Synonyms bolA homolog 1 (E. coli), BolA like protein 1, BOLA1, CGI 143, hBolA, RP11 196G18.18
Calculated Molecular Weight 137 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC063405
Gene Symbol BOLA1
Gene ID (NCBI) 51027
RRIDAB_2066933
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3E2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BOLA1 antibody 18017-1-APDownload protocol
IHC protocol for BOLA1 antibody 18017-1-APDownload protocol
IF protocol for BOLA1 antibody 18017-1-APDownload protocol
IP protocol for BOLA1 antibody 18017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biomed Res Int

Prognostic Values of BolA Family Member Expression in Hepatocellular Carcinoma

Authors - Dong Wang
View Article
mouseWB

bioRxiv

Proteome profiling identifies a link between the mitochondrial pathways and host-microbial sensor ELMO1 following Salmonella infection

Authors - Sajan C Achi
View Article