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  • Featured Product
  • KD/KO Validated

BOP1 Recombinant antibody, PBS Only

BOP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A4

Cat no : 82742-3-PBS

Print datasheet

Synonyms

KIAA0124, block of proliferation 1, 3A4

验证数据展示

  • WB analysis using 82742-3-RR (same clone as 82742-3-PBS)

    WB analysis using 82742-3-RR (same clone as 82742-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82742-3-RR (BOP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82742-3-RR (same clone as 82742-3-PBS)

    WB analysis of HeLa using 82742-3-RR (same clone as 82742-3-PBS)

    WB result of BOP1 antibody (82742-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BOP1 transfected HeLa cells. This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 82742-3-RR (same clone as 82742-3-PBS)

    IHC staining of human ovarian cancer using 82742-3-RR (same clone as 82742-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82742-3-RR (BOP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BOP1 antibody included
  • IHC staining of human ovarian cancer using 82742-3-RR (same clone as 82742-3-PBS)

    IHC staining of human ovarian cancer using 82742-3-RR (same clone as 82742-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82742-3-RR (BOP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BOP1 antibody included
  • IF Staining of HepG2 using 82742-3-RR (same clone as 82742-3-PBS)

    IF Staining of HepG2 using 82742-3-RR (same clone as 82742-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BOP1 antibody (82742-3-RR, Clone: 3A4 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82742-3-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy BOP1 Ready-To-Use IHC Kit. BOP1 primary antibody included.

产品信息

82742-3-PBS targets BOP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen BOP1 fusion protein Ag26669 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name block of proliferation 1
Synonyms KIAA0124, block of proliferation 1, 3A4
Calculated Molecular Weight 84 kDa
Observed Molecular Weight 117 kDa
GenBank Accession NumberBC013787
Gene Symbol BOP1
Gene ID (NCBI) 23246
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14137
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Block of Proliferation 1(BOP1) protein was first isolated from a mouse, and the truncation protein may led to cell cycle arrest. Bop1 plays a role in processing of the 28S and 5.8S rRNAs (ribosomal RNAs) to regulate ribosome biogenesis. And Bop1 also act as a component of Pes1/Bop1/WDR21 (PeBoW) complex for mature 60S ribosome, and consequently acts during cell division at G1 checkpoints (PMID:18670790; PMID:26940494). What's more, abnormal expression of BOP1 was associated with liver or colorectal cancers (PMID:16804918). The molecular weight of the protein we detected was slightly higher, about 117 kd, which is also seen in the article (PMID: 30100995).