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验证数据展示

  • WB analysis using 82742-3-RR

    WB analysis using 82742-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82742-3-RR (BOP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82742-3-RR

    WB analysis of HeLa using 82742-3-RR

    WB result of BOP1 antibody (82742-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BOP1 transfected HeLa cells.

  • IHC staining of human ovarian cancer using 82742-3-RR

    IHC staining of human ovarian cancer using 82742-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82742-3-RR (BOP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BOP1 antibody included
  • IHC staining of human ovarian cancer using 82742-3-RR

    IHC staining of human ovarian cancer using 82742-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82742-3-RR (BOP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BOP1 antibody included
  • IF Staining of HepG2 using 82742-3-RR

    IF Staining of HepG2 using 82742-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BOP1 antibody (82742-3-RR, Clone: 3A4 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

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  • KD/KO验证

BOP1 Recombinant antibody

BOP1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82742-3-RR

产品说明书

CloneNo. 3A4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

KIAA0124, block of proliferation 1, 3A4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inNCI-H1299 cells, HeLa cells, DU 145 cells, PC-3 cells, U2OS celss
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy BOP1 Ready-To-Use IHC Kit. BOP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82742-3-RR targets BOP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 BOP1 fusion protein Ag26669 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 block of proliferation 1
别名 KIAA0124, block of proliferation 1, 3A4
计算分子量 84 kDa
观测分子量 117 kDa
GenBank蛋白编号BC013787
基因名称 BOP1
Gene ID (NCBI) 23246
RRIDAB_3086524
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14137
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Block of Proliferation 1(BOP1) protein was first isolated from a mouse, and the truncation protein may led to cell cycle arrest. Bop1 plays a role in processing of the 28S and 5.8S rRNAs (ribosomal RNAs) to regulate ribosome biogenesis. And Bop1 also act as a component of Pes1/Bop1/WDR21 (PeBoW) complex for mature 60S ribosome, and consequently acts during cell division at G1 checkpoints (PMID:18670790; PMID:26940494). What's more, abnormal expression of BOP1 was associated with liver or colorectal cancers (PMID:16804918). The molecular weight of the protein we detected was slightly higher, about 117 kd, which is also seen in the article (PMID: 30100995).

实验方案

Product Specific Protocols
WB protocol for BOP1 antibody 82742-3-RRDownload protocol
IHC protocol for BOP1 antibody 82742-3-RRDownload protocol
IF protocol for BOP1 antibody 82742-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols