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  • Featured Product
  • KD/KO Validated

BOP1 Recombinant antibody

BOP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3A4

Cat no : 82742-3-RR

Print datasheet

Synonyms

KIAA0124, block of proliferation 1, 3A4

验证数据展示

  • WB analysis using 82742-3-RR

    WB analysis using 82742-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82742-3-RR (BOP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82742-3-RR

    WB analysis of HeLa using 82742-3-RR

    WB result of BOP1 antibody (82742-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BOP1 transfected HeLa cells.

  • IHC staining of human ovarian cancer using 82742-3-RR

    IHC staining of human ovarian cancer using 82742-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82742-3-RR (BOP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BOP1 antibody included
  • IHC staining of human ovarian cancer using 82742-3-RR

    IHC staining of human ovarian cancer using 82742-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82742-3-RR (BOP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BOP1 antibody included
  • IF Staining of HepG2 using 82742-3-RR

    IF Staining of HepG2 using 82742-3-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BOP1 antibody (82742-3-RR, Clone: 3A4 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

经过测试的应用

Positive WB detected inNCI-H1299 cells, HeLa cells, DU 145 cells, PC-3 cells, U2OS celss
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy BOP1 Ready-To-Use IHC Kit. BOP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82742-3-RR targets BOP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen BOP1 fusion protein Ag26669 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name block of proliferation 1
Synonyms KIAA0124, block of proliferation 1, 3A4
Calculated Molecular Weight 84 kDa
Observed Molecular Weight 117 kDa
GenBank Accession NumberBC013787
Gene Symbol BOP1
Gene ID (NCBI) 23246
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14137
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Block of Proliferation 1(BOP1) protein was first isolated from a mouse, and the truncation protein may led to cell cycle arrest. Bop1 plays a role in processing of the 28S and 5.8S rRNAs (ribosomal RNAs) to regulate ribosome biogenesis. And Bop1 also act as a component of Pes1/Bop1/WDR21 (PeBoW) complex for mature 60S ribosome, and consequently acts during cell division at G1 checkpoints (PMID:18670790; PMID:26940494). What's more, abnormal expression of BOP1 was associated with liver or colorectal cancers (PMID:16804918). The molecular weight of the protein we detected was slightly higher, about 117 kd, which is also seen in the article (PMID: 30100995).

实验方案

Product Specific Protocols
WB protocol for BOP1 antibody 82742-3-RRDownload protocol
IHC protocol for BOP1 antibody 82742-3-RRDownload protocol
IF protocol for BOP1 antibody 82742-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols