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- KD/KO Validated
CoraLite® Plus 488-conjugated BOP1 Recombinant antibody
BOP1 Uni-rAbTM Recombinant Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3A4
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy BOP1 Ready-To-Use IHC Kit. BOP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82742-3-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82742-3 targets BOP1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | BOP1 fusion protein Ag26669 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | block of proliferation 1 |
| Synonyms | |
| Calculated Molecular Weight | 84 kDa |
| Observed Molecular Weight | 117 kDa |
| GenBank Accession Number | BC013787 |
| Gene Symbol | BOP1 |
| Gene ID (NCBI) | 23246 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14137 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Block of Proliferation 1(BOP1) protein was first isolated from a mouse, and the truncation protein may led to cell cycle arrest. Bop1 plays a role in processing of the 28S and 5.8S rRNAs (ribosomal RNAs) to regulate ribosome biogenesis. And Bop1 also act as a component of Pes1/Bop1/WDR21 (PeBoW) complex for mature 60S ribosome, and consequently acts during cell division at G1 checkpoints (PMID:18670790; PMID:26940494). What's more, abnormal expression of BOP1 was associated with liver or colorectal cancers (PMID:16804918). The molecular weight of the protein we detected was slightly higher, about 117 kd, which is also seen in the article (PMID: 30100995).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 BOP1 antibody CL488-82742-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |