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CoraLite®594-conjugated BORIS Monoclonal antibody

BORIS Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B12E2

Cat no : CL594-60079

Print datasheet

Synonyms

BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, dJ579F20.2, Zinc finger protein CTCF T

验证数据展示

  • IF Staining of HeLa using CL594-60079

    IF Staining of HeLa using CL594-60079

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 BORIS antibody (CL594-60079, Clone: 2B12E2 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CTCFL/BORIS Ready-To-Use IHC Kit. CTCFL/BORIS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60079-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60079 targets BORIS in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen BORIS fusion protein Ag1003 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CCCTC-binding factor (zinc finger protein)-like
Synonyms BORIS, Cancer/testis antigen 27, CCCTC binding factor, CT27, CTCF like protein, CTCF paralog, CTCF T, CTCFL, dJ579F20.2, Zinc finger protein CTCF T
Calculated Molecular Weight 82 kDa, 75 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC130486
Gene Symbol BORIS/CTCFL
Gene ID (NCBI) 140690
RRIDAB_2883418
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NI51
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTCFL, also named BORIS (for brother of the regulator of imprinted sites) is a paralogue of the 11 zinc-finger transcription factor, CTCF. It is predominantly expressed in spermatocytes in the testis, also can be found in tumors and cancer cell lines. It mainly participates in insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. It has a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2.

实验方案

Product Specific Protocols
IF protocol for CL594 BORIS antibody CL594-60079Download protocol
Standard Protocols
Click here to view our Standard Protocols