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BRAP Recombinant antibody, PBS Only

BRAP Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240099D8

Cat no : 83314-6-PBS

Print datasheet

Synonyms

Renal carcinoma antigen NY-REN-63, IMP, EC:2.3.2.27, BRCA1-associated protein, BRCA1 associated protein

验证数据展示

  • IF Staining of MCF-7 using 83314-6-RR (same clone as 83314-6-PBS)

    IF Staining of MCF-7 using 83314-6-RR (same clone as 83314-6-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using BRAP antibody (83314-6-RR, Clone: 240099D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83314-6-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83314-6-RR (same clone as 83314-6-PBS)

    IF Staining of HeLa using 83314-6-RR (same clone as 83314-6-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BRAP antibody (83314-6-RR, Clone: 240099D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83314-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83314-6-RR (same clone as 83314-6-PBS)

    FC experiment of HeLa using 83314-6-RR (same clone as 83314-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BRAP Recombinant antibody (83314-6-RR, Clone:240099D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83314-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83314-6-RR

    Affinity and Kinetic Characterization of 83314-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83314-6-RR against Human BRAP were performed. The affinity constant is 6.34 nM.

产品信息

83314-6-PBS targets BRAP in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen BRAP fusion protein Ag26728 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name BRCA1 associated protein
Synonyms Renal carcinoma antigen NY-REN-63, IMP, EC:2.3.2.27, BRCA1-associated protein, BRCA1 associated protein
Calculated Molecular Weight 592 aa, 67 kDa
GenBank Accession NumberBC136698
Gene Symbol BRAP
Gene ID (NCBI) 8315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ7Z569
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C