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验证数据展示

  • IF Staining of MCF-7 using 83314-6-RR

    IF Staining of MCF-7 using 83314-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using BRAP antibody (83314-6-RR, Clone: 240099D8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IF Staining of HeLa using 83314-6-RR

    IF Staining of HeLa using 83314-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BRAP antibody (83314-6-RR, Clone: 240099D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83314-6-RR

    FC experiment of HeLa using 83314-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BRAP Recombinant antibody (83314-6-RR, Clone:240099D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83314-6-RR

    Affinity and Kinetic Characterization of 83314-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83314-6-RR against Human BRAP were performed. The affinity constant is 6.34 nM.

BRAP Recombinant antibody

BRAP Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83314-6-RR

产品说明书

CloneNo. 240099D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Renal carcinoma antigen NY-REN-63, IMP, EC:2.3.2.27, BRCA1-associated protein, BRCA1 associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83314-6-RR targets BRAP in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 BRAP fusion protein Ag26728 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BRCA1 associated protein
别名 Renal carcinoma antigen NY-REN-63, IMP, EC:2.3.2.27, BRCA1-associated protein, BRCA1 associated protein
计算分子量 592 aa, 67 kDa
GenBank蛋白编号BC136698
基因名称 BRAP
Gene ID (NCBI) 8315
RRIDAB_3670980
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ7Z569
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for BRAP antibody 83314-6-RRDownload protocol
FC protocol for BRAP antibody 83314-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols