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BRAP Recombinant antibody

BRAP Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240099D8

Cat no : 83314-6-RR

Print datasheet

Synonyms

Renal carcinoma antigen NY-REN-63, IMP, EC:2.3.2.27, BRCA1-associated protein, BRCA1 associated protein

验证数据展示

  • IF Staining of MCF-7 using 83314-6-RR

    IF Staining of MCF-7 using 83314-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using BRAP antibody (83314-6-RR, Clone: 240099D8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • FC experiment of HeLa using 83314-6-RR

    FC experiment of HeLa using 83314-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BRAP Recombinant antibody (83314-6-RR, Clone:240099D8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83314-6-RR

    Affinity and Kinetic Characterization of 83314-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83314-6-RR against Human BRAP were performed. The affinity constant is 6.34 nM.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83314-6-RR is BRAP Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen BRAP fusion protein Ag26728 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name BRCA1 associated protein
Synonyms Renal carcinoma antigen NY-REN-63, IMP, EC:2.3.2.27, BRCA1-associated protein, BRCA1 associated protein
Calculated Molecular Weight 592 aa, 67 kDa
GenBank Accession NumberBC136698
Gene Symbol BRAP
Gene ID (NCBI) 8315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ7Z569
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for BRAP antibody 83314-6-RRDownload protocol
FC protocol for BRAP antibody 83314-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols