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验证数据展示

  • WB analysis using 68922-1-Ig

    WB analysis using 68922-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68922-1-Ig (BRD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 68922-1-Ig

    FC experiment of HeLa using 68922-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.2 μg BRD9 Monoclonal antibody (68922-1-Ig, Clone:1C10F2, red) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1), and 0.2 μg Mouse IgG1 isotype Control Antibody (66360-1-Ig, Clone: T1F8D3F10, blue) was stained parallely as control. Cells were fixed with 4% PFA .

BRD9 Monoclonal antibody

BRD9 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68922-1-Ig

Print datasheet

CloneNo. 1C10F2

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

UNQ3040/PRO9856, Rhabdomyosarcoma antigen MU-RMS-40.8, LAVS3040, Bromodomain-containing protein 9, BRD 9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, MCF-7 cells, MDA-MB-231 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68922-1-Ig targets BRD9 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen BRD9 fusion protein Ag20438 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name bromodomain containing 9
Synonyms UNQ3040/PRO9856, Rhabdomyosarcoma antigen MU-RMS-40.8, LAVS3040, Bromodomain-containing protein 9, BRD 9
Calculated Molecular Weight 597 aa, 67 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC041590
Gene Symbol BRD9
Gene ID (NCBI) 65980
RRIDAB_3670453
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H8M2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRD9, also named as MU-RMS-40.8, may play a role in chromatin remodeling and regulation of transcription.

实验方案

Product Specific Protocols
WB protocol for BRD9 antibody 68922-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols