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验证数据展示

  • WB analysis using 68922-1-Ig

    WB analysis using 68922-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68922-1-Ig (BRD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 68922-1-Ig

    FC experiment of HeLa using 68922-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.2 μg BRD9 Monoclonal antibody (68922-1-Ig, Clone:1C10F2, red) and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L) (Cat.NO. SA00013-1), and 0.2 μg Mouse IgG1 isotype Control Antibody (66360-1-Ig, Clone: T1F8D3F10, blue) was stained parallely as control. Cells were fixed with 4% PFA .

BRD9 Monoclonal antibody

BRD9 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 68922-1-Ig

产品说明书

CloneNo. 1C10F2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

UNQ3040/PRO9856, Rhabdomyosarcoma antigen MU-RMS-40.8, LAVS3040, Bromodomain-containing protein 9, BRD 9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, MCF-7 cells, MDA-MB-231 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68922-1-Ig targets BRD9 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 BRD9 fusion protein Ag20438 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 bromodomain containing 9
别名 UNQ3040/PRO9856, Rhabdomyosarcoma antigen MU-RMS-40.8, LAVS3040, Bromodomain-containing protein 9, BRD 9
计算分子量 597 aa, 67 kDa
观测分子量70 kDa
GenBank蛋白编号BC041590
基因名称 BRD9
Gene ID (NCBI) 65980
RRIDAB_3670453
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9H8M2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRD9, also named as MU-RMS-40.8, may play a role in chromatin remodeling and regulation of transcription.

实验方案

Product Specific Protocols
WB protocol for BRD9 antibody 68922-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols