CoraLite® Plus 488-conjugated BRN2 Polyclonal antibody
BRN2 Polyclonal Antibody for IF-P
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy POU3F2/BRN2 Ready-To-Use IHC Kit. POU3F2/BRN2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 14596-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-14596 targets BRN2 in IF-P applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | BRN2 fusion protein Ag6131 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | POU class 3 homeobox 2 |
| Synonyms | Brain 2, Brn 2, BRN2, Oct 7, OCT7, Octamer binding protein 7, OTF 7, OTF7, POU class 3 homeobox 2, POU3F2, POUF3 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC051699 |
| Gene Symbol | BRN2 |
| Gene ID (NCBI) | 5454 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20265 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. It shares a highly homologous region that referred to as the POU domain. POU3F2 is a Class III POU genes that expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression. POU3F2 is required for maintaining neural cell differetiation and has a role in the production and positioning of neocortical neurons.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 BRN2 antibody CL488-14596 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |