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验证数据展示

  • WB analysis using 30186-1-AP

    WB analysis using 30186-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30186-1-AP (BRPF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 30186-1-AP

    FC experiment of HeLa using 30186-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human BRPF1 (30186-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HeLa using 30186-1-AP

    IF Staining of HeLa using 30186-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BRPF1 antibody (30186-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

BRPF1 Polyclonal antibody

BRPF1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 30186-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Protein Br140, Peregrin, Bromodomain and PHD finger-containing protein 1, BR140

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, PC-12 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30186-1-AP targets BRPF1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, rat
Immunogen BRPF1 fusion protein Ag32828 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bromodomain and PHD finger containing, 1
Synonyms Protein Br140, Peregrin, Bromodomain and PHD finger-containing protein 1, BR140
Calculated Molecular Weight 1220 aa, 138 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC053851
Gene Symbol BRPF1
Gene ID (NCBI) 7862
RRIDAB_2935526
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55201
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRPF1 (bromodomain and PHD finger-containing protein 1) is also named as BR140. BRPF1 is scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PMID:16387653, PMID:24065767, PMID:27939640). BRPF1 plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac) (PMID:24065767). And BRPF1 positively regulates the transcription of RUNX1 and RUNX2 (PMID:18794358).

实验方案

Product Specific Protocols
WB protocol for BRPF1 antibody 30186-1-APDownload protocol
IF protocol for BRPF1 antibody 30186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols