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验证数据展示

  • FC experiment of HeLa using CL488-30186

    FC experiment of HeLa using CL488-30186

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Brpf1 Polyclonal Antibody (CL488-30186)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated BRPF1 Polyclonal antibody

BRPF1 Polyclonal Antibody for FC (Intra)
Cat No. CL488-30186

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

FC (Intra)

Synonyms

Protein Br140, Peregrin, Bromodomain and PHD finger-containing protein 1, BR140

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 30186-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-30186 targets BRPF1 in FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, rat
Immunogen BRPF1 fusion protein Ag32828 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name bromodomain and PHD finger containing, 1
Synonyms Protein Br140, Peregrin, Bromodomain and PHD finger-containing protein 1, BR140
Calculated Molecular Weight 1220 aa, 138 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC053851
Gene Symbol BRPF1
Gene ID (NCBI) 7862
RRIDAB_3672844
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55201
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BRPF1 (bromodomain and PHD finger-containing protein 1) is also named as BR140. BRPF1 is scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PMID:16387653, PMID:24065767, PMID:27939640). BRPF1 plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac) (PMID:24065767). And BRPF1 positively regulates the transcription of RUNX1 and RUNX2 (PMID:18794358).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 BRPF1 antibody CL488-30186Download protocol
Standard Protocols
Click here to view our Standard Protocols