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验证数据展示

  • FC experiment of Ramos using CL594-60349

    FC experiment of Ramos using CL594-60349

    1X10^6 Ramos cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human BSAP,PAX5 (CL594-60349, Clone:2H2D6) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated BSAP,PAX5 Monoclonal antibody

BSAP,PAX5 Monoclonal Antibody for FC (Intra)
Cat No. CL594-60349

产品说明书

CloneNo. 2H2D6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC (Intra)

别名

BSAP, BSAP,PAX5, paired box 5, Paired box protein Pax 5, PAX5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inRamos cells
Positive FC detected inRamos cells
For other applications, we recommend the unconjugated version of this antibody, 60349-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60349 targets BSAP,PAX5 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 BSAP,PAX5 fusion protein Ag16023 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 paired box 5
别名 BSAP, BSAP,PAX5, paired box 5, Paired box protein Pax 5, PAX5
计算分子量 391 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC156927
基因名称 PAX5
Gene ID (NCBI) 5079
RRIDAB_2934711
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ02548
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members.

实验方案

Product Specific Protocols
FC protocol for CL594 BSAP,PAX5 antibody CL594-60349Download protocol
Standard Protocols
Click here to view our Standard Protocols