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BSEP Monoclonal antibody, PBS Only

BSEP Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse , Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3C11D5

Cat No : 67512-1-PBS

Print datasheet

Synonyms

ABC16, ABCB11, Bile salt export pump, BRIC2, BSEP, PFIC 2, PFIC2, PGY4, SPGP

验证数据展示

  • WB analysis using 67512-1-Ig (same clone as 67512-1-PBS)

    WB analysis using 67512-1-Ig (same clone as 67512-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    WB analysis of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67512-1-Ig (BSEP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    IHC staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    IHC staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67512-1-Ig (BSEP antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    IF Staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BSEP antibody (67512-1-Ig, Clone: 3C11D5 ) at dilution of 1:10000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    IF Staining of mouse liver using 67512-1-Ig (same clone as 67512-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using BSEP antibody (67512-1-Ig, Clone: 3C11D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67512-1-PBS in a different storage buffer formulation.

产品信息

67512-1-PBS targets BSEP in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Tested Reactivity Human, Mouse , Rat
Immunogen BSEP fusion protein Ag29135 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 11
Synonyms ABC16, ABCB11, Bile salt export pump, BRIC2, BSEP, PFIC 2, PFIC2, PGY4, SPGP
Calculated Molecular Weight 146 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberNM_003742
Gene Symbol ABCB11
Gene ID (NCBI) 8647
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO95342
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

ABCB11, also named as BSEP, belongs to the ABC transporter superfamily. ABCB11 involved in the ATP-dependent secretion of bile salts into the canaliculus of hepatocytes. Mutation of ABCB11 will cause the progressive familial intrahepatic cholestasis type 2 (PFIC2) and the benign recurrent intrahepatic cholestasis type 2 (BRIC2). Genetic variations in ABCB11 may play a role in drug-induced cholestasis causing liver damage. The calculated MW of ABCB11 is 146 kDa, 67512-1-Ig can detect bands around 150 kDa. It's reported that ABCB11 has several N-glycosylation sites, the observed higher molecular mass may due to posttranslational N-glycosylation. (PMID: 9545351, 24359682)