BSEP Monoclonal antibody
BSEP Monoclonal Antibody for WB, IF-P, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse , Rat, pig and More (1)
Applications
WB, IF-P, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3C11D5
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, unboiled HepG2 cells, rat liver tissue, mouse liver tissue, pig lung tissue, HeLa cells, A549 cells |
Positive IHC detected in | mouse liver tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse liver tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:8000-1:32000 |
Immunofluorescence (IF) | IF : 1:5000-1:20000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
67512-1-Ig targets BSEP in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse , Rat, pig samples.
Tested Applications | WB, IF-P, IHC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | Human, Mouse , Rat, pig |
Cited Reactivity | mouse |
Immunogen | BSEP fusion protein Ag29135 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | ATP-binding cassette, sub-family B (MDR/TAP), member 11 |
Synonyms | ABC16, ABCB11, Bile salt export pump, BRIC2, BSEP, PFIC 2, PFIC2, PGY4, SPGP |
Calculated Molecular Weight | 146 kDa |
Observed Molecular Weight | 150-160 kDa |
GenBank Accession Number | NM_003742 |
Gene Symbol | ABCB11 |
Gene ID (NCBI) | 8647 |
RRID | AB_2882733 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | O95342 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for BSEP antibody 67512-1-Ig | Download protocol |
IHC protocol for BSEP antibody 67512-1-Ig | Download protocol |
IF protocol for BSEP antibody 67512-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Pharmacol Vitamin C and vitamin D3 alleviate metabolic-associated fatty liver disease by regulating the gut microbiota and bile acid metabolism via the gut-liver axis | ||
Appl Biochem Biotechnol Sphincter of Oddi Dysfunction Induces Gallstone by Inhibiting the Expression of ABCB11 via PKC-α |