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  • Featured Product
  • KD/KO Validated

BUB1 Polyclonal antibody, PBS Only

BUB1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13330-1-PBS

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Synonyms

验证数据展示

  • IF Staining of IMR-90 cells using 13330-1-AP (same clone as 13330-1-PBS)

    IF Staining of IMR-90 cells using 13330-1-AP (same clone as 13330-1-PBS)

    IF results of BUB1 anitbody with IMR-90 cells by M.S. Grasiella Angelina Andriani Cristina Montagna Lab at Albert Einstein College of Medicine-Bronx - NY. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

  • WB analysis of MKN-45 using 13330-1-AP (same clone as 13330-1-PBS)

    WB analysis of MKN-45 using 13330-1-AP (same clone as 13330-1-PBS)

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

  • WB analysis using 13330-1-AP (same clone as 13330-1-PBS)

    WB analysis using 13330-1-AP (same clone as 13330-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

产品信息

13330-1-PBS targets BUB1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, canine samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, canine
Immunogen BUB1 fusion protein Ag4138 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name budding uninhibited by benzimidazoles 1 homolog (yeast)
Synonyms
Calculated Molecular Weight 1085 aa, 122 kDa
Observed Molecular Weight 45 kDa, 120 kDa
GenBank Accession NumberBC028201
Gene Symbol BUB1
Gene ID (NCBI) 699
RRIDAB_2814999
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43683
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).