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验证数据展示

  • IF Staining of IMR-90 cells using 13330-1-AP (same clone as 13330-1-PBS)

    IF Staining of IMR-90 cells using 13330-1-AP (same clone as 13330-1-PBS)

    IF results of BUB1 anitbody with IMR-90 cells by M.S. Grasiella Angelina Andriani Cristina Montagna Lab at Albert Einstein College of Medicine-Bronx - NY. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

  • WB analysis of MKN-45 using 13330-1-AP (same clone as 13330-1-PBS)

    WB analysis of MKN-45 using 13330-1-AP (same clone as 13330-1-PBS)

    MKN-45 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

  • WB analysis using 13330-1-AP (same clone as 13330-1-PBS)

    WB analysis using 13330-1-AP (same clone as 13330-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 13330-1-AP (BUB1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13330-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

BUB1 Polyclonal antibody, PBS Only

BUB1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 13330-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, canine

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

13330-1-PBS targets BUB1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, canine samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, canine
免疫原 BUB1 fusion protein Ag4138 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 budding uninhibited by benzimidazoles 1 homolog (yeast)
别名
计算分子量 1085 aa, 122 kDa
观测分子量 45 kDa, 120 kDa
GenBank蛋白编号BC028201
基因名称 BUB1
Gene ID (NCBI) 699
RRIDAB_2814999
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43683
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Bub1 is an evolutionarily conserved mitotic checkpoint control protein that is present in diverse organisms including yeast and humans. Bub1 is a serine/threonine protein kinase and is required for recruitment of Mad1, Mad2, Bub3, and CENP-E to kinetochores. Full-length Bub1 protein was cleaved to an 45 kDa fragment and an intermediate fragment of 90 kDa, only detected at early stages of apoptosis execution, indicating cleavage of Bub1 in the amino- and carboxy-terminal regions of the protein(PMID:15818396).