• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

BUB1B Monoclonal antibody, PBS Only

BUB1B Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B2D5

Cat no : 60423-1-PBS

Print datasheet

Synonyms

BubR1, hBUBR1, EC:2.7.11.1, BUB1beta, Bub1A

验证数据展示

  • WB analysis using 60423-1-Ig (same clone as 60423-1-PBS)

    WB analysis using 60423-1-Ig (same clone as 60423-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60423-1-Ig (BUB1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60423-1-PBS in a different storage buffer formulation.

  • WB analysis using 60423-1-Ig (same clone as 60423-1-PBS)

    WB analysis using 60423-1-Ig (same clone as 60423-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60423-1-Ig (BUB1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60423-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy BUB1B Ready-To-Use IHC Kit. BUB1B primary antibody included.

产品信息

60423-1-PBS targets BUB1B in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human
Immunogen BUB1B fusion protein Ag34182 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name budding uninhibited by benzimidazoles 1 homolog beta (yeast)
Synonyms BubR1, hBUBR1, EC:2.7.11.1, BUB1beta, Bub1A
Calculated Molecular Weight 1050 aa, 120 kDa
Observed Molecular Weight120-130 kDa
GenBank Accession NumberBC018739
Gene Symbol BubR1
Gene ID (NCBI) 701
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO60566
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B are the cause of premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).