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BUB3 Recombinant antibody, PBS Only

BUB3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240087D9

Cat no : 83266-5-PBS

Print datasheet

Synonyms

hBUB3, BUB3L, BUB 3, 240087D9

验证数据展示

  • WB analysis using 83266-5-RR (same clone as 83266-5-PBS)

    WB analysis using 83266-5-RR (same clone as 83266-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83266-5-RR (BUB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    IF Staining of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    FC experiment of HeLa using 83266-5-RR (same clone as 83266-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BUB3 Recombinant antibody (83266-5-RR, Clone:240087D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83266-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83266-5-RR

    Affinity and Kinetic Characterization of 83266-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83266-5-RR against Human BUB3 were performed. The affinity constant is 0.228 nM.

Planning an IHC experiment? We recommend our IHCeasy BUB3 Ready-To-Use IHC Kit. BUB3 primary antibody included.

产品信息

83266-5-PBS targets BUB3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, rat
Immunogen BUB3 fusion protein Ag25608 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name budding uninhibited by benzimidazoles 3 homolog (yeast)
Synonyms hBUB3, BUB3L, BUB 3, 240087D9
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC005138
Gene Symbol BUB3
Gene ID (NCBI) 9184
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO43684
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C