• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83266-5-RR

    WB analysis using 83266-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83266-5-RR (BUB3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 83266-5-RR

    IF Staining of HeLa using 83266-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 83266-5-RR

    IF Staining of HeLa using 83266-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 83266-5-RR

    IF Staining of HeLa using 83266-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BUB3 antibody (83266-5-RR, Clone: 240087D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 83266-5-RR

    FC experiment of HeLa using 83266-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug BUB3 Recombinant antibody (83266-5-RR, Clone:240087D9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83266-5-RR

    Affinity and Kinetic Characterization of 83266-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83266-5-RR against Human BUB3 were performed. The affinity constant is 0.228 nM.

BUB3 Recombinant antibody

BUB3 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83266-5-RR

产品说明书

CloneNo. 240087D9

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, FC (Intra), ELISA

别名

hBUB3, BUB3L, BUB 3, 240087D9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, MCF-7 cells, Jurkat cells, C6 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy BUB3 Ready-To-Use IHC Kit. BUB3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83266-5-RR targets BUB3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, rat
免疫原 BUB3 fusion protein Ag25608 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 budding uninhibited by benzimidazoles 3 homolog (yeast)
别名 hBUB3, BUB3L, BUB 3, 240087D9
计算分子量 37 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC005138
基因名称 BUB3
Gene ID (NCBI) 9184
RRIDAB_3670939
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO43684
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for BUB3 antibody 83266-5-RRDownload protocol
IF protocol for BUB3 antibody 83266-5-RRDownload protocol
FC protocol for BUB3 antibody 83266-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols