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验证数据展示

  • IHC staining of human colon cancer using 83330-2-RR

    IHC staining of human colon cancer using 83330-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83330-2-RR (Bcl-XL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with BCL2L1 antibody included
  • IF Staining of HeLa using 83330-2-RR

    IF Staining of HeLa using 83330-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Bcl-xL antibody (83330-2-RR, Clone: 240450A9 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • FC experiment of HeLa using 83330-2-RR

    FC experiment of HeLa using 83330-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Bcl-xL Recombinant antibody (83330-2-RR, Clone:240450A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Bcl-XL Recombinant antibody

Bcl-XL Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83330-2-RR

产品说明书

CloneNo. 240450A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells, Jurkat cells
Planning an IHC experiment? We recommend our IHCeasy BCL2L1 Ready-To-Use IHC Kit. BCL2L1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83330-2-RR targets Bcl-XL in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Bcl-XL fusion protein Ag25604 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 BCL2-like 1
别名 BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X, Apoptosis regulator Bcl X
计算分子量 26 kDa
GenBank蛋白编号BC019307
基因名称 Bcl-XL
Gene ID (NCBI) 598
RRIDAB_3670994
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ07817
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L), Bcl-X(s), and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-X(L) isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can form heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-X(s) isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator. Bcl-X(beta) is a 227 amino acid protein.

实验方案

Product Specific Protocols
IHC protocol for Bcl-XL antibody 83330-2-RRDownload protocol
IF protocol for Bcl-XL antibody 83330-2-RRDownload protocol
FC protocol for Bcl-XL antibody 83330-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols