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Bcl2 Polyclonal antibody

Bcl2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 26593-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat and More (6)

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)

Bcl-2, Bcl 2, B cell leukemia/lymphoma 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inNIH/3T3 cells, C2C12 cells, mouse brain tissue, mouse heart tissue, mouse testis tissue, mouse kidney tissue, mouse spleen tissue, rat spleen tissue, rat kidney tissue, rat testis tissue, mouse colon tissue
Positive IHC detected inmouse spleen tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26593-1-AP targets Bcl2 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 mouse, rat
文献引用反应性mouse, rat, pig, canine, chicken, bovine, hamster, duck
免疫原 Bcl2 fusion protein Ag24260 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 B-cell leukemia/lymphoma 2
别名 Bcl-2, Bcl 2, B cell leukemia/lymphoma 2
计算分子量 26 kDa
观测分子量 26 kDa
GenBank蛋白编号NM-009741
基因名称 Bcl2
Gene ID (NCBI) 12043
RRIDAB_2818996
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1.       What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2.       FAQs for Bcl-2

  1.  How to measure Bcl-2 and Bax apoptotic markers by western blotting

    Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.

  2. What band size should I expect in western blotting for Bcl-2?

    The molecular weight of Bcl-2 is 26 kDa.


实验方案

Product Specific Protocols
WB protocol for Bcl2 antibody 26593-1-APDownload protocol
IHC protocol for Bcl2 antibody 26593-1-APDownload protocol
IF protocol for Bcl2 antibody 26593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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ACS Nano

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Acta Pharm Sin B

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Authors - Yujun Zhou
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Redox Biol

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Authors - Zhe Wang
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Clin Transl Med

PINK1 modulates Prdx2 to reduce lipotoxicity-induced apoptosis and attenuate cardiac dysfunction in heart failure mice with a preserved ejection fraction

Authors - Hao Zhang
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J Nanobiotechnology

PD-L1 blockade TAM-dependently potentiates mild photothermal therapy against triple-negative breast cancer

Authors - Chao Wang
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Adv Healthc Mater

GDNF-Loaded Polydopamine Nanoparticles-Based Anisotropic Scaffolds Promote Spinal Cord Repair by Modulating Inhibitory Microenvironment

Authors - Jinjin Ma
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