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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Bcl2 Polyclonal antibody

Bcl2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26593

Print datasheet

Synonyms

Bcl-2, Bcl 2, B cell leukemia/lymphoma 2

验证数据展示

  • IF Staining of NIH/3T3 using CL488-26593

    IF Staining of NIH/3T3 using CL488-26593

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 Bcl2 antibody (CL488-26593) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 26593-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26593 targets Bcl2 in IF/ICC applications and shows reactivity with mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity mouse, rat
Immunogen Bcl2 fusion protein Ag24260 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name B-cell leukemia/lymphoma 2
Synonyms Bcl-2, Bcl 2, B cell leukemia/lymphoma 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberNM-009741
Gene Symbol Bcl2
Gene ID (NCBI) 12043
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1.       What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2.       FAQs for Bcl-2

  1.  How to measure Bcl-2 and Bax apoptotic markers by western blotting

    Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.

  2. What band size should I expect in western blotting for Bcl-2?

    The molecular weight of Bcl-2 is 26 kDa.


实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Bcl2 antibody CL488-26593Download protocol
Standard Protocols
Click here to view our Standard Protocols