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验证数据展示

  • IF Staining of NIH/3T3 using CL488-26593

    IF Staining of NIH/3T3 using CL488-26593

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 Bcl2 antibody (CL488-26593) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Bcl2 Polyclonal antibody

Bcl2 Polyclonal Antibody for IF/ICC
Cat No. CL488-26593

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

IF/ICC

别名

Bcl-2, Bcl 2, B cell leukemia/lymphoma 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 26593-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26593 targets Bcl2 in IF/ICC applications and shows reactivity with mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 mouse, rat
免疫原 Bcl2 fusion protein Ag24260 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 B-cell leukemia/lymphoma 2
别名 Bcl-2, Bcl 2, B cell leukemia/lymphoma 2
计算分子量 26 kDa
观测分子量 26 kDa
GenBank蛋白编号NM-009741
基因名称 Bcl2
Gene ID (NCBI) 12043
RRIDAB_3672801
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

1.       What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2.       FAQs for Bcl-2

  1.  How to measure Bcl-2 and Bax apoptotic markers by western blotting

    Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.

  2. What band size should I expect in western blotting for Bcl-2?

    The molecular weight of Bcl-2 is 26 kDa.


实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Bcl2 antibody CL488-26593Download protocol
Standard Protocols
Click here to view our Standard Protocols