Bcl2 Recombinant antibody

Bcl2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82469-6-RR

产品说明书

CloneNo. 240571H5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, FC (Intra), ELISA

Bcl-2, Bcl 2, B cell leukemia/lymphoma 2, 240571H5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse kidney tissue, mouse spleen tissue, mouse heart tissue
Positive FC (Intra) detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82469-6-RR targets Bcl2 in WB, FC (Intra), ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 mouse
免疫原 Bcl2 fusion protein Ag24260 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell leukemia/lymphoma 2
别名 Bcl-2, Bcl 2, B cell leukemia/lymphoma 2, 240571H5
计算分子量 26 kDa
观测分子量26 kDa
GenBank蛋白编号NM-009741
基因名称 Bcl2
Gene ID (NCBI) 12043
RRIDAB_3670523
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP10417
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

实验方案

Product Specific Protocols
WB protocol for Bcl2 antibody 82469-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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