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Mouse Bcl2 Recombinant antibody, PBS Only

Bcl2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240571H5

Cat no : 82469-6-PBS

Print datasheet

Synonyms

Bcl-2, Bcl 2, B cell leukemia/lymphoma 2, 240571H5

验证数据展示

  • WB analysis using 82469-6-RR (same clone as 82469-6-PBS)

    WB analysis using 82469-6-RR (same clone as 82469-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82469-6-RR (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82469-6-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 82469-6-RR (same clone as 82469-6-PBS)

    FC experiment of NIH/3T3 using 82469-6-RR (same clone as 82469-6-PBS)

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug Bcl2 Recombinant antibody (82469-6-RR, Clone:240571H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82469-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82469-6-RR

    Affinity and Kinetic Characterization of 82469-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82469-6-RR against Mouse Bcl2 were performed. The affinity constant is 1.08 nM.

产品信息

82469-6-PBS targets Bcl2 in WB, FC (Intra), ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity mouse
Immunogen Bcl2 fusion protein Ag24260 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name B-cell leukemia/lymphoma 2
Synonyms Bcl-2, Bcl 2, B cell leukemia/lymphoma 2, 240571H5
Calculated Molecular Weight 26 kDa
GenBank Accession NumberNM-009741
Gene Symbol Bcl2
Gene ID (NCBI) 12043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10417
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C