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Mouse Bcl2 Recombinant antibody, PBS Only

Bcl2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240571H5

Cat no : 82469-6-PBS

Print datasheet

Synonyms

Bcl-2, Bcl 2, B cell leukemia/lymphoma 2, 240571H5

验证数据展示

  • WB analysis using 82469-6-RR (same clone as 82469-6-PBS)

    WB analysis using 82469-6-RR (same clone as 82469-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82469-6-RR (Bcl2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82469-6-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 82469-6-RR (same clone as 82469-6-PBS)

    FC experiment of NIH/3T3 using 82469-6-RR (same clone as 82469-6-PBS)

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug Bcl2 Recombinant antibody (82469-6-RR, Clone:240571H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82469-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82469-6-RR

    Affinity and Kinetic Characterization of 82469-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82469-6-RR against Mouse Bcl2 were performed. The affinity constant is 1.08 nM.

产品信息

The immunogen of 82469-6-PBS is Bcl2 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity mouse
Immunogen Bcl2 fusion protein Ag24260 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name B-cell leukemia/lymphoma 2
Synonyms Bcl-2, Bcl 2, B cell leukemia/lymphoma 2, 240571H5
Calculated Molecular Weight 26 kDa
GenBank Accession NumberNM-009741
Gene Symbol Bcl2
Gene ID (NCBI) 12043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP10417
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C