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验证数据展示

  • IF Staining of NIH/3T3 using CL488-68103

    IF Staining of NIH/3T3 using CL488-68103

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite® Plus 488 Bcl2 antibody (CL488-68103, Clone: 1B3F7 ) at dilution of 1:100.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Bcl2 Monoclonal antibody

Bcl2 Monoclonal Antibody for IF/ICC
Cat No. CL488-68103

Print datasheet

CloneNo. 1B3F7

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat

Applications

IF/ICC

Synonyms

Bcl2, Bcl-2, 1B3F7, B cell leukemia/lymphoma 2, Bcl 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 68103-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68103 targets Bcl2 in IF/ICC applications and shows reactivity with mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity mouse, rat
Immunogen Bcl2 fusion protein Ag24261 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name B-cell leukemia/lymphoma 2
Synonyms Bcl2, Bcl-2, 1B3F7, B cell leukemia/lymphoma 2, Bcl 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberNM-009741
Gene Symbol Bcl2
Gene ID (NCBI) 12043
RRIDAB_3084401
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Bcl2 antibody CL488-68103Download protocol
Standard Protocols
Click here to view our Standard Protocols