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验证数据展示

  • IF Staining of NIH/3T3 using CL555-66799

    IF Staining of NIH/3T3 using CL555-66799

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using CoraLite®555 Bcl2 antibody (CL555-66799, Clone: 1B5B9 ) at dilution of 1:200.

  • FC experiment of NIH/3T3 using CL555-66799

    FC experiment of NIH/3T3 using CL555-66799

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug CoraLite®555-conjugated Bcl2 Monoclonal antibody (CL555-66799, Clone:1B5B9)(red), or 0.5 ug CoraLite® Plus 555 Mouse IgG1 Isotype Control (1F8D3) (CL555-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®555-conjugated Bcl2 Monoclonal antibody

Bcl2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-66799

Print datasheet

CloneNo. 1B5B9

Host / Isotype

Mouse / IgG1

Reactivity

mouse

Applications

IF/ICC, FC (Intra)

Synonyms

Bcl-2, Bcl 2, B cell leukemia/lymphoma 2, 1B5B9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®555
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66799 targets Bcl2 in IF/ICC, FC (Intra) applications and shows reactivity with mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity mouse
Immunogen Bcl2 fusion protein Ag24261 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name B-cell leukemia/lymphoma 2
Synonyms Bcl-2, Bcl 2, B cell leukemia/lymphoma 2, 1B5B9
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberNM-009741
Gene Symbol Bcl2
Gene ID (NCBI) 12043
RRIDAB_2919696
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

实验方案

Product Specific Protocols
IF protocol for CL555 Bcl2 antibody CL555-66799Download protocol
Standard Protocols
Click here to view our Standard Protocols