验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HeLa cells, MCF-7 cells |
Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68502-1-Ig targets Beta Arrestin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | Beta Arrestin 2 fusion protein Ag29807 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | arrestin, beta 2 |
别名 | ARRB2, β-Arrestin 2, BARR2, Arrestin beta-2, ARR2 |
计算分子量 | 46 kDa |
观测分子量 | 50 kDa |
GenBank蛋白编号 | BC007427 |
基因名称 | Beta Arrestin 2 |
Gene ID (NCBI) | 409 |
RRID | AB_3085213 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P32121 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta Arrestin 2 is a member of arrestin/beta-arrestin protein family, which are expressed at high levels in the central nervous system (CNS) and play a critical role in the regulation of G-protein coupled receptors (GPCRs) including MOR. Beta Arrestin 2 is an adaptor protein that is important for the regulation of receptors belonging to both dopaminergic and opioid systems. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors.
实验方案
Product Specific Protocols | |
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WB protocol for Beta Arrestin 2 antibody 68502-1-Ig | Download protocol |
IHC protocol for Beta Arrestin 2 antibody 68502-1-Ig | Download protocol |
IF protocol for Beta Arrestin 2 antibody 68502-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |