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  • Featured Product
  • KD/KO Validated

Beta Catenin Polyclonal antibody, PBS Only

Beta Catenin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17565-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 17565-1-AP (same clone as 17565-1-PBS)

    WB analysis using 17565-1-AP (same clone as 17565-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17565-1-AP (Beta Catenin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 17565-1-AP (same clone as 17565-1-PBS)

    IHC staining of human liver cancer using 17565-1-AP (same clone as 17565-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IHC staining of human liver cancer using 17565-1-AP (same clone as 17565-1-PBS)

    IHC staining of human liver cancer using 17565-1-AP (same clone as 17565-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IP experiment of HEK-293 using 17565-1-AP (same clone as 17565-1-PBS)

    IP experiment of HEK-293 using 17565-1-AP (same clone as 17565-1-PBS)

    IP result of anti-Beta Catenin (IP:17565-1-AP, 4ug; Detection:17565-1-AP 1:5000) with HEK-293 cells lysate 1920 ug. This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 17565-1-AP (same clone as 17565-1-PBS)

    IF Staining of MCF-7 using 17565-1-AP (same clone as 17565-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, green). This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 17565-1-AP (same clone as 17565-1-PBS)

    FC experiment of MCF-7 using 17565-1-AP (same clone as 17565-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Beta Catenin (17565-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17565-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.

产品信息

17565-1-PBS targets Beta Catenin in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Beta Catenin fusion protein Ag11763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Synonyms
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC058926
Gene Symbol Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2088102
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35222
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)