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Beta Catenin Polyclonal antibody
Beta Catenin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)
Conjugate
Unconjugated
116
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, NIH/3T3 cells, HeLa cells, mouse brain tissue, A431 cells, MCF-7 cells, mouse liver tissue, rat brain tissue, C6 cells, rat liver tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells, T-47D cells, U-251 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17565-1-AP targets Beta Catenin in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Beta Catenin fusion protein Ag11763 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
| Synonyms | CTNNB, Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin |
| Calculated Molecular Weight | 781 aa, 86 kDa |
| Observed Molecular Weight | 92 kDa |
| GenBank Accession Number | BC058926 |
| Gene Symbol | Beta Catenin |
| Gene ID (NCBI) | 1499 |
| ENSEMBL Gene ID | ENSG00000168036 |
| RRID | AB_2088102 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35222 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta Catenin antibody 17565-1-AP | Download protocol |
| IHC protocol for Beta Catenin antibody 17565-1-AP | Download protocol |
| IF protocol for Beta Catenin antibody 17565-1-AP | Download protocol |
| IP protocol for Beta Catenin antibody 17565-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest JAM3 maintains leukemia-initiating cell self-renewal through LRP5/AKT/β-catenin/CCND1 signaling. | ||
Cell Death Dis USP8 positively regulates hepatocellular carcinoma tumorigenesis and confers ferroptosis resistance through β-catenin stabilization | ||
Mater Today Bio A novel adhesive dual-sensitive hydrogel for sustained release of exosomes derived from M2 macrophages promotes repair of bone defects | ||
Br J Pharmacol Reregulation of hepatic stellate cell contraction and cirrhotic portal hypertension by Wnt/β-catenin signaling via interaction with Gli1. | ||
Acta Pharmacol Sin Identification of HMGCR as the anticancer target of physapubenolide against melanoma cells by in silico target prediction. | ||
