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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Beta Catenin Polyclonal antibody

Beta Catenin Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-17565

Print datasheet

Synonyms

CTNNB, Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin

验证数据展示

  • IF Staining of MCF-7 using CL594-17565

    IF Staining of MCF-7 using CL594-17565

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 Beta Catenin antibody (CL594-17565) at dilution of 1:200.

  • FC experiment of MCF-7 using CL594-17565

    FC experiment of MCF-7 using CL594-17565

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Beta Catenin (CL594-17565) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inMCF-7 cells, T-47D cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17565-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-17565 targets Beta Catenin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Beta Catenin fusion protein Ag11763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Synonyms CTNNB, Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC058926
Gene Symbol Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2919845
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35222
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

实验方案

Product Specific Protocols
IF protocol for CL594 Beta Catenin antibody CL594-17565Download protocol
Standard Protocols
Click here to view our Standard Protocols