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验证数据展示

  • IF Staining of HepG2 using CL488-66586

    IF Staining of HepG2 using CL488-66586

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 Beta Galactosidase antibody (CL488-66586, Clone: 4F4F4 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Beta Galactosidase Monoclonal antibody

Beta Galactosidase Monoclonal Antibody for IF/ICC
Cat No. CL488-66586

Print datasheet

CloneNo. 4F4F4

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Synonyms

Acid beta galactosidase, Beta galactosidase, EBP, Elastin receptor 1, ELNR1, galactosidase, beta 1, GLB1, Lactase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66586-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66586 targets Beta Galactosidase in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat
Immunogen Beta Galactosidase fusion protein Ag8069 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name galactosidase, beta 1
Synonyms Acid beta galactosidase, Beta galactosidase, EBP, Elastin receptor 1, ELNR1, galactosidase, beta 1, GLB1, Lactase
Calculated Molecular Weight 76 kDa
Observed Molecular Weight 64-66 kDa, 76-85 kDa
GenBank Accession NumberBC007493
Gene Symbol GLB1/Beta-galactosidase
Gene ID (NCBI) 2720
RRIDAB_2919348
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16278
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda and 61 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Beta Galactosidase antibody CL488-66586Download protocol
Standard Protocols
Click here to view our Standard Protocols