验证数据展示
产品信息
83706-6-PBS targets Beta galactosidase as part of a matched antibody pair:
MP00647-4: 83706-4-PBS capture and 83706-6-PBS detection (validated in Sandwich ELISA)
Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.
This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.
经测试应用 | WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test Application Description |
经测试反应性 | human |
免疫原 | Beta galactosidase fusion protein Ag7792 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | galactosidase, beta 1 |
别名 | GLB1, ELNR1, EC:3.2.1.23, EBP, Beta-galactosidase |
计算分子量 | 76 kDa |
观测分子量 | 62-67 kDa |
GenBank蛋白编号 | BC007493 |
基因名称 | GLB1/Beta-galactosidase |
Gene ID (NCBI) | 2720 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P16278 |
储存缓冲液 | PBS only , pH 7.3 |
储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). The MW of GLB1 after glycosylation is 100-120 kd. GLB1 is prone to produce homodimers (220-240 kd) and higher multimers (PMID: 3926488). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda, and 61 kDa.