验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, SH-SY5Y cells, HEK-293 cells, K-562 cells |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83706-6-RR targets Beta galactosidase in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Beta galactosidase fusion protein Ag7792 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | galactosidase, beta 1 |
| 别名 | GLB1, ELNR1, EC:3.2.1.23, EBP, Beta-galactosidase |
| 计算分子量 | 76 kDa |
| 观测分子量 | 62-67 kDa |
| GenBank蛋白编号 | BC007493 |
| 基因名称 | GLB1/Beta-galactosidase |
| Gene ID (NCBI) | 2720 |
| RRID | AB_3671308 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P16278 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). The MW of GLB1 after glycosylation is 100-120 kd. GLB1 is prone to produce homodimers (220-240 kd) and higher multimers (PMID: 3926488). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda, and 61 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Beta galactosidase antibody 83706-6-RR | Download protocol |
| IF protocol for Beta galactosidase antibody 83706-6-RR | Download protocol |
| FC protocol for Beta galactosidase antibody 83706-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |