CoraLite® Plus 488-conjugated Beta galactosidase Recombinant antibody

Beta galactosidase Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-83706-6

产品说明书

CloneNo. 240788F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

GLB1, ELNR1, EC:3.2.1.23, EBP, Beta-galactosidase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 83706-6-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83706-6 targets Beta galactosidase in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Beta galactosidase fusion protein Ag7792 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 galactosidase, beta 1
别名 GLB1, ELNR1, EC:3.2.1.23, EBP, Beta-galactosidase
计算分子量 76 kDa
观测分子量 62-67 kDa
GenBank蛋白编号BC007493
基因名称 GLB1/Beta-galactosidase
Gene ID (NCBI) 2720
RRIDAB_3673292
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP16278
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLB1(Beta-galactosidase) is also named as ELNR1 or Lactase. It cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. This protein is identical to the elastin-binding protein (EBP), a major component of the nonintegrin cell surface receptor complex expressed in fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1), GM1-gangliosidosis type 2 (GM1G2), GM1-gangliosidosis type 3 (GM1G3) and mucopolysaccharidosis type 4B (MPS4B). GBL1 is synthesized as an 85-kDa precursor that is C-terminally processed into a 64-66 kDa mature form and the released ~20-kDa proteolytic fragment was thought to be degraded (PMID: 10744681). The MW of GLB1 after glycosylation is 100-120 kd. GLB1 is prone to produce homodimers (220-240 kd) and higher multimers (PMID: 3926488). GLB1 has 3 isoforms with MW of 76 kDa, 73 kda, and 61 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Beta galactosidase antibody CL488-83706-6Download protocol
Standard Protocols
Click here to view our Standard Protocols
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