• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Brefeldin A

Brefeldin A (Cyanein) 是一种布雷菲尔德青霉代谢物，它是一种大环内酯，具有广泛的抗生素活性。 它还是 HCT 116 细胞中蛋白质转运的 ATPase 抑制剂(IC50=0.2 μM)，诱导 Y 细胞分化和凋亡。

CAS号

20350-15-6

分子式

C16H24O4

主要靶点

CRISPR/Cas9|Autophagy|HSV|Antibiotic|ATPase|Mitophagy

仅限科研使用

Cat No : CM03062

Print datasheet

Synonyms

Ascotoxin|布雷非德菌素 A|BFA|Decumbin|Cyanein

验证数据展示

  • CAS No.: 20350-15-6

    Brefeldin A
    CAS#: 20350-15-6

产品信息

Brefeldin A , a Penicillium brefeldianum metabolite, which is a macrocyclic lactone exhibiting a wide range of antibiotic activity. It is also an ATPase inhibitor for protein transport in HCT 116 cells(IC50=0.2 μM), inducing Y cell differentiation and apoptosis.

CAS号 20350-15-6
分子式 C16H24O4
主要靶点 CRISPR/Cas9|Autophagy|HSV|Antibiotic|ATPase|Mitophagy
主要通路 DNA损伤和修复|离子通道|微生物学|自噬
分子量 280.36
纯度 99.26%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year
别名 Ascotoxin|布雷非德菌素 A|BFA|Decumbin|Cyanein

靶点活性

ATPase (HCT 116):0.2 μM

体内活性

在HF4.9 和 HF28RA 细胞中, Brefeldin A（25 ng/mL）完全抑制细胞生长。在HF1A3 细胞中,Brefeldin A (75 ng/mL) 完全抑制细胞生长。

溶解度

Ethanol:2.8 mg/mL (10 mM),DMSO:14 mg/mL (50 mM)

细胞实验

HF1A3, HF4.9 cell viability upon the treatments is tested using double staining of cells with YO-PRO 1/PI and SYTO16/PI probes. To access cell proliferation, cells are treated with 0–100 ng/mL Brefeldin A in complete medium for 20 hours before adding 1 μCi/mL [methyl-3H]-thymidine for additional 4 hours at 37 °C. The incorporated radioactive thymidine is quantified by scintillation counting with Microbeta counter. To examine long-term effects of Brefeldin A treatment, cells are seeded at initial concentration 105 cells/mL and treated with 0-75 ng/mL Brefeldin A for up to 5 days. At the time indicated, a sample of cells is removed and viable cell number is assessed by standard Trypan Blue exclusion assay.(Only for Reference)

参考文献

1.Zhu JW, et al. Bioorg Med Chem. 2000, 8(2), 455-463.
2.Ohnishi Y, et al. Br J Pharmacol. 2001, 134(1), 168-178.
3.Franco M, et al. J Biol Chem. 1996, 271(3), 1573-1578.
4.Hullin-Matsuda F, et al. J Biol Chem. 2012.
5.Wlodkowic D, et al. Leuk Res. 2007, 31(12), 1687-1700.
6.Wang J, et al. Erythroleukemia cells acquire an alternative mitophagy capability. Sci Rep. 2016 Apr 19;6:24641.
7.Zhang Y, Hu H, Liu W, et al. Amino acids and RagD potentiate mTORC1 activation in CD8+ T cells to confer antitumor immunity[J]. Journal for ImmunoTherapy of Cancer. 2021, 9(4): e002137.
8.Bai R, Li L, Liu M, et al. Paper Based 3D Scaffold for Multiplexed Single Cell Secretomic Analysis[J]. Analytical chemistry. 2018, 90(9): 5825-5832.
9.Ma X, Zou F, Yu F, et al. Nanoparticle Vaccines Based on the Receptor Binding Domain (RBD) and Heptad Repeat (HR) of SARS-CoV-2 Elicit Robust Protective Immune Responses[J]. Immunity. 2020

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
=
×
×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
×
=
×
C1   V1   C2   V2