Brefeldin A

Brefeldin A inhibits protein translocation from the endoplasmic reticulum (ER) to the Golgi apparatus reversibly, can be used to induce autophagy in mammalian cells.

CAS号

20350-15-6

分子式

C16H24O4

主要靶点

ATPase

仅限科研使用

Cat No : CM03062

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Synonyms

Ascotoxin; Cyanein; Decumbin; Nectrolide



产品信息

Brefeldin A is a fungal lactone antibiotic produced by Penicillium brefeldianum usually used as a protein trafficking/secretion inhibitor in mammalian and other eukaryotic cells, without significant effect on protein synthesis. The main target of Brefeldin A may be Arf1, which is important to formation of protein transport vesicles[1][2][3]. Brefeldin A could induce the rapid redistribution of the ll0-kD protein and the movement of Golgi membrane into the ER within 15min at 2μg/ml in semipermeable cells[4]. Brefeldin A could induce a wide variety of human cancer cells to differentiation and apoptosis. It exhibited cytotoxicity to HCT 116 cells with IC50 value of 0.2μM, as well as induced DNA fragmentation and apoptotic morphological changes with IC50 values of 0.11 and 0.36 μM, respectively[5]. Brefeldin A combined with L755507 could improve the HDR efficiency, and exhibit enhanced insertion efficiency by 2-fold, with maximal effect at 0.1 μM[6].

CAS号 20350-15-6
分子式 C16H24O4
主要靶点 ATPase
主要通路 Membrane Transporter/Ion Channel
分子量 280.3594
MDL NO MFCD00083258
Pubchem ID
纯度 99.88%, 此纯度可做参考,具体纯度与批次有关系,可咨询客服
储存条件
粉末 -20°C 3年
液体 -80°C 1年
运输条件 Shipped in cold pack
别名 Ascotoxin; Cyanein; Decumbin; Nectrolide

靶点

TargetActivity
ATPaseIC50=0.2μM

溶解度

DMSO50 mg/mL
Waterinsoluble

细胞研究

Cell LinesConcentrationAssay TypeTimeActivity DescriptionData Sources
3T3-L15 μg/mlFunction Assay30 minrecapitulates insulin action with respect to regulating Akt activity and AS160 phosphorylation24843827
3T3-L15 μg/mlFunction Assay30 mincauses reversible redistribution of GLUT424843827
3T3-L15 μg/mlFunction Assay30 minmimics the effects of insulin and causes robust phosphorylation of Akt (Ser 473) and phosphorylation of AS160 (Thr 642 and Ser 588)24843827
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动物研究

剂量Mice: 26.3 mg/kg[7] (i.v.), LD50 = 250 mg/kg[8] (i.p.)
给药途径i.v., i.p.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
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C1   V1   C2   V2