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Brefeldin A

CAS号

20350-15-6

分子式

C16H24O4

主要靶点

CRISPR/Cas9|Autophagy|HSV|Antibiotic|ATPase|Mitophagy

仅限科研使用

Cat No : CM03062

Print datasheet

Synonyms

Herpes simplex virus|HSV|inhibit|Decumbin|CRISPR/Cas9|CRISPR|Cyanein|Ascotoxin|Antibiotic|Cas9|Brefeldin A|ATPase (HCT 116)|Autophagy|BFA|布雷非德菌素 A|布雷菲德菌素 A|Mitochondrial Autophagy|Mitophagy|Inhibitor

验证数据展示

  • CAS No.: 20350-15-6

    Brefeldin A
    CAS#: 20350-15-6

产品信息

CAS号 20350-15-6
分子式 C16H24O4
主要靶点 CRISPR/Cas9|Autophagy|HSV|Antibiotic|ATPase|Mitophagy
主要通路 微生物学|自噬|离子通道|自噬|微生物学|DNA 损伤和修复
分子量 280.36
纯度 99.89%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
别名 Herpes simplex virus|HSV|inhibit|Decumbin|CRISPR/Cas9|CRISPR|Cyanein|Ascotoxin|Antibiotic|Cas9|Brefeldin A|ATPase (HCT 116)|Autophagy|BFA|布雷非德菌素 A|布雷菲德菌素 A|Mitochondrial Autophagy|Mitophagy|Inhibitor

靶点活性

ATPase (HCT 116 cells):0.2 μM

体内活性

方法：为检测体内抗肿瘤活性，将 Brefeldin A (15 mg/kg in 10% ricinus oil+5% DMSO+10% ethanol+75% physiologic saline) 腹腔注射给携带人结直肠癌肿瘤 HCT116 的 BALB/c 小鼠，每天一次，持续四周。 结果：Brefeldin A 在体内抑制 HCT116 肿瘤生长。[3] 方法：为检测体内抗肿瘤活性，将 Brefeldin A (16-64 mg/kg in distilled water containing 0.05% Tween 80) 腹腔注射给携带肿瘤 LOX IMVI 的 Athymic NCr nu/nu 小鼠，每天两次，持续五天。 结果：Brefeldin A 在体内显示出抗肿瘤活性，小鼠寿命增加 65%-100%，第 60 天幸存者增加 17%-50%。[4]

体外活性

方法：肿瘤细胞 HL60 、K562 和 HT-29 用 Brefeldin A (2 μM) 处理 72 h，使用 DNA filter elution assay 检测 DNA 片段。 结果：Brefeldin A 以不同的动力学诱导 DNA 断裂。HL60 细胞在 15 h 内观察到完整的 DNA 片段，而 K562 和 HT-29 细胞则需要 48-72 h。[1] 方法：人乳腺癌细胞 MDA-MB-231 用 Brefeldin A (0.05-1 μg/mL) 处理 24 h，使用 Western Blot 方法检测靶点蛋白表达水平。 结果：PARP 切割是细胞死亡的标志性事件，可以在 Brefeldin A 处理的悬浮 MDA-MB-231 细胞中检测到。[2]

溶解度

Ethanol:2.8 mg/mL (10 mM);DMSO:14 mg/mL (50 mM)

细胞实验

HF1A3, HF4.9 cell viability upon the treatments is tested using double staining of cells with YO-PRO 1/PI and SYTO16/PI probes. To access cell proliferation, cells are treated with 0–100 ng/mL Brefeldin A in complete medium for 20 hours before adding 1 μCi/mL [methyl-3H]-thymidine for additional 4 hours at 37 °C. The incorporated radioactive thymidine is quantified by scintillation counting with Microbeta counter. To examine long-term effects of Brefeldin A treatment, cells are seeded at initial concentration 105 cells/mL and treated with 0-75 ng/mL Brefeldin A for up to 5 days. At the time indicated, a sample of cells is removed and viable cell number is assessed by standard Trypan Blue exclusion assay.(Only for Reference)

参考文献

1.Shao RG, et al. Brefeldin A is a potent inducer of apoptosis in human cancer cells independently of p53. Exp Cell Res. 1996 Sep 15;227(2):190-6.
2.Tseng CN, et al. Brefeldin A reduces anchorage-independent survival, cancer stem cell potential and migration of MDA-MB-231 human breast cancer cells. Molecules. 2014 Oct 29;19(11):17464-77.
3.Zhou L, et al. Brefeldin A inhibits colorectal cancer growth by triggering Bip/Akt-regulated autophagy. FASEB J. 2019 Apr;33(4):5520-5534.
4.Sausville EA, et al. Antiproliferative effect in vitro and antitumor activity in vivo of brefeldin A. Cancer J Sci Am.
5.Wlodkowic D, et al. Leuk Res. 2007, 31(12), 1687-1700.
6.Wang J, et al. Erythroleukemia cells acquire an alternative mitophagy capability. Sci Rep. 2016 Apr 19;6:24641.
7.Zhang Y, Hu H, Liu W, et al. Amino acids and RagD potentiate mTORC1 activation in CD8+ T cells to confer antitumor immunity[J]. Journal for ImmunoTherapy of Cancer. 2021, 9(4): e002137.
8.Bai R, Li L, Liu M, et al. Paper Based 3D Scaffold for Multiplexed Single Cell Secretomic Analysis[J]. Analytical chemistry. 2018, 90(9): 5825-5832.
9.Ma X, Zou F, Yu F, et al. Nanoparticle Vaccines Based on the Receptor Binding Domain (RBD) and Heptad Repeat (HR) of SARS-CoV-2 Elicit Robust Protective Immune Responses[J]. Immunity. 2020

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