Brefeldin A

Brefeldin A (Cyanein) 是一种布雷菲尔德青霉代谢物,它是一种大环内酯,具有广泛的抗生素活性。 它还是 HCT 116 细胞中蛋白质转运的 ATPase 抑制剂(IC50=0.2 μM),诱导 Y 细胞分化和凋亡。

CAS号

20350-15-6

分子式

C16H24O4

主要靶点

CRISPR/Cas9|Autophagy|HSV|Antibiotic|ATPase|Mitophagy

仅限科研使用

Cat No : CM03062

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Synonyms

Ascotoxin|布雷非德菌素 A|BFA|Decumbin|Cyanein



产品信息

Brefeldin A , a Penicillium brefeldianum metabolite, which is a macrocyclic lactone exhibiting a wide range of antibiotic activity. It is also an ATPase inhibitor for protein transport in HCT 116 cells(IC50=0.2 μM), inducing Y cell differentiation and apoptosis.

CAS号 20350-15-6
分子式 C16H24O4
主要靶点 CRISPR/Cas9|Autophagy|HSV|Antibiotic|ATPase|Mitophagy
主要通路 DNA损伤和修复|离子通道|微生物学|自噬
分子量 280.36
纯度 99.26%, 此纯度可做参考,具体纯度与批次有关系,可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year
别名 Ascotoxin|布雷非德菌素 A|BFA|Decumbin|Cyanein

靶点活性

ATPase (HCT 116):0.2 μM

体内活性

在HF4.9 和 HF28RA 细胞中, Brefeldin A(25 ng/mL)完全抑制细胞生长。在HF1A3 细胞中,Brefeldin A (75 ng/mL) 完全抑制细胞生长。

溶解度

Ethanol:2.8 mg/mL (10 mM),DMSO:14 mg/mL (50 mM)

细胞实验

HF1A3, HF4.9 cell viability upon the treatments is tested using double staining of cells with YO-PRO 1/PI and SYTO16/PI probes. To access cell proliferation, cells are treated with 0–100 ng/mL Brefeldin A in complete medium for 20 hours before adding 1 μCi/mL [methyl-3H]-thymidine for additional 4 hours at 37 °C. The incorporated radioactive thymidine is quantified by scintillation counting with Microbeta counter. To examine long-term effects of Brefeldin A treatment, cells are seeded at initial concentration 105 cells/mL and treated with 0-75 ng/mL Brefeldin A for up to 5 days. At the time indicated, a sample of cells is removed and viable cell number is assessed by standard Trypan Blue exclusion assay.(Only for Reference)

参考文献

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