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C12orf29 Recombinant antibody

C12orf29 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242163C3

Cat no : 84708-1-RR

Print datasheet

Synonyms

Rnl, RNA Ligase 1, RNA Ligase, 242163C3

验证数据展示

  • WB analysis of HeLa using 84708-1-RR

    WB analysis of HeLa using 84708-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 84708-1-RR (C12orf29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84708-1-RR

    Affinity and Kinetic Characterization of 84708-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84708-1-RR against Human C12orf29 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, U-87 MG cells, LNCaP cells, K-562 cells, rat pancreas tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84708-1-RR targets C12orf29 in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, rat
Immunogen C12orf29 fusion protein Ag35681 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromosome 12 open reading frame 29
Synonyms Rnl, RNA Ligase 1, RNA Ligase, 242163C3
Calculated Molecular Weight37kDa，325aa
Observed Molecular Weight37 kDa
GenBank Accession NumberNM_001009894.2
Gene Symbol C12orf29
Gene ID (NCBI) 91298
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N999
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C12orf29, also known as RLIG1, is a 37 kDa human protein consisting of 325 amino acids. It catalyzes ATP-dependent RNA ligation via a three-step mechanism involving tandem auto- and RNA AMPylation (PMID: 36792600).

实验方案

Product Specific Protocols
WB protocol for C12orf29 antibody 84708-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols