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验证数据展示

  • WB analysis using 84320-2-RR (same clone as 84320-2-PBS)

    WB analysis using 84320-2-RR (same clone as 84320-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84320-2-RR (C14orf133 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84320-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01194-2

    Cytometric bead array standard curve of MP01194-2

    Cytometric bead array standard curve of MP01194-2, VIPAS39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84320-2-PBS. Detection antibody: 84320-3-PBS. Standard: Ag14762. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01194-3

    Cytometric bead array standard curve of MP01194-3

    Cytometric bead array standard curve of MP01194-3, VIPAS39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84320-2-PBS. Detection antibody: 84320-1-PBS. Standard: Ag14762. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84320-2-RR

    Affinity and Kinetic Characterization of 84320-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84320-2-RR against Human VIPAS39 were performed. The affinity constant is below 1 pM.

VIPAS39 Recombinant antibody, PBS Only (Capture)

VIPAS39 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 84320-2-PBS

产品说明书

CloneNo. 241616A12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, Cytometric bead array, Indirect ELISA

别名

C14orf133, 241616A12, hSPE 39, hSPE-39, SPE39

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84320-2-PBS targets VIPAS39 as part of a matched antibody pair:

MP01194-2: 84320-2-PBS capture and 84320-3-PBS detection (validated in Cytometric bead array)

MP01194-3: 84320-2-PBS capture and 84320-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 VIPAS39 fusion protein Ag14762 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 14 open reading frame 133
别名 C14orf133, 241616A12, hSPE 39, hSPE-39, SPE39
计算分子量 493 aa, 57 kDa
观测分子量50 kDa
GenBank蛋白编号BC015054
基因名称 VIPAS39
Gene ID (NCBI) 63894
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H9C1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VIPAS39 (also known as C14orf133 or SPE-39) is a binding protein to Vps33B, one of the subunits in the mammalian HOPS complex. VIPAS39 may be involved in endosomal maturation or fusion (PMID: 23918659). Mutations in Vps33B and VIPAS39 cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a rare autosomal recessive multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).